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Related Concept Videos

Mechanical Protein Functions01:58

Mechanical Protein Functions

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Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force. 
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Mechanical Protein Function01:58

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Transducer Mechanism: G Protein–Coupled Receptors01:30

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G Protein–Coupled Receptors (GPCRs) are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to various stimuli. GPCRs regulate critical physiological pathways and are excellent drug targets for treating diseases such as diabetes, cancer, obesity, depression, or Alzheimer's. Nearly 35% of approved drugs implement their therapeutic effects by selectively interacting with specific GPCRs.
GPCRs are also called heptahelical,...
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Protein-Drug Binding: Mechanism and Kinetics01:16

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Protein-drug binding refers to the interaction between drugs and proteins within the body. This binding process can occur intracellularly, involving drug interactions with enzymes or receptors within cells, or extracellularly, involving plasma proteins in the blood.
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Aggregates Classification01:29

Aggregates Classification

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Aggregate classification is generally based on its size, petrographic characteristics, weight, and source. Size classification ranges from coarse to fine aggregates, defined by the size of the particles. Coarse aggregates are particles that do not pass through ASTM sieve No. 4, and aggregates that pass through the sieve are fine aggregates.
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Combinatorial Gene Control02:33

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Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
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Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans
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Protein aggregation - Mechanisms, detection, and control.

Wei Wang1, Christopher J Roberts2

  • 1Biological Development, Bayer USA, LLC, 800 Dwight Way, Berkeley, CA 94710, United States.

International Journal of Pharmaceutics
|August 27, 2018
PubMed
Summary
This summary is machine-generated.

Protein aggregation is a major hurdle for protein drugs, affecting quality and safety. This review covers aggregation mechanisms, influencing factors, detection, and control strategies.

Keywords:
AggregateColloidal stabilityConformationOligomerizationOpalescenceParticulate

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Area of Science:

  • Biopharmaceutical Development
  • Protein Chemistry
  • Drug Product Quality

Background:

  • Protein aggregation poses significant challenges in developing and commercializing protein-based therapeutics.
  • Aggregates can negatively impact product quality, safety, and efficacy throughout manufacturing, storage, and administration.

Purpose of the Study:

  • To provide a comprehensive review of protein aggregation in the context of biopharmaceutical development.
  • To summarize key aspects including aggregation mechanisms, influencing factors, detection methods, and control strategies.

Main Methods:

  • Literature review of recent scientific publications on protein aggregation.
  • Synthesis of information on four core areas: mechanisms, factors, detection, and control.

Main Results:

  • Detailed discussion of the various mechanisms driving protein aggregation.
  • Identification of critical factors that influence protein aggregate formation.
  • Overview of current and emerging techniques for detecting protein aggregates.
  • Exploration of strategies for controlling and preventing protein aggregation.

Conclusions:

  • Understanding and managing protein aggregation is crucial for successful biopharmaceutical development.
  • A multi-faceted approach combining knowledge of mechanisms, factors, detection, and control is essential.