Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.9K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.9K
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

14.8K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
14.8K
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

5.5K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
5.5K
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

7.3K
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
7.3K
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

5.5K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
5.5K
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

4.4K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
4.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Telemedicine for international travelers through a Smartphone-based monitoring platform (Trip Doctor®).

Travel medicine and infectious disease·2022
Same author

High serum nitrates levels in non-survivor COVID-19 patients.

Medicina intensiva·2022
Same author

Validation of medical researchs.

Medicina intensiva·2022
Same author

Blood caspase-8 concentrations and mortality among septic patients.

Medicina intensiva·2022
Same author

New findings of edible oil characterization by ultrasonic parameters.

Food chemistry·2021
Same author

Benefica chirurgia. A global surgery project focusing on hernia surgery.

The surgeon : journal of the Royal Colleges of Surgeons of Edinburgh and Ireland·2021

Related Experiment Video

Updated: Feb 6, 2026

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
13:28

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

Published on: May 16, 2017

50.9K

A new algorithm for high-resolution reconstruction of single particles by electron microscopy.

C O S Sorzano1, J Vargas2, J M de la Rosa-Trevín3

  • 1Centro Nac. Biotecnología (CSIC), 28049 Cantoblanco, Madrid, Spain; Univ. San Pablo - CEU, Campus Urb. Montepríncipe, 28668 Boadilla del Monte, Madrid, Spain.

Journal of Structural Biology
|August 27, 2018
PubMed
Summary

A new Xmipp high resolution reconstruction protocol (highres) in Scipion improves cryo-EM map quality. This advanced method enhances feature detection for higher resolution structural analysis.

Keywords:
3D reconstructionAngular assignmentImage processingVolume restoration

More Related Videos

Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method
12:10

Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method

Published on: March 28, 2011

24.0K
Author Spotlight: Optimizing Cryo-EM Analysis with CryoSieve for Enhanced Particle Selection Efficiency
06:41

Author Spotlight: Optimizing Cryo-EM Analysis with CryoSieve for Enhanced Particle Selection Efficiency

Published on: May 10, 2024

2.6K

Related Experiment Videos

Last Updated: Feb 6, 2026

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
13:28

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

Published on: May 16, 2017

50.9K
Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method
12:10

Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method

Published on: March 28, 2011

24.0K
Author Spotlight: Optimizing Cryo-EM Analysis with CryoSieve for Enhanced Particle Selection Efficiency
06:41

Author Spotlight: Optimizing Cryo-EM Analysis with CryoSieve for Enhanced Particle Selection Efficiency

Published on: May 10, 2024

2.6K

Area of Science:

  • Structural Biology
  • Biophysics
  • Computational Biology

Background:

  • The Electron Microscopy Data Bank's Map Challenge spurred innovation in cryo-electron microscopy (cryo-EM) data processing.
  • Existing reconstruction protocols have limitations in achieving the highest possible resolution.

Purpose of the Study:

  • To introduce and detail a novel high-resolution reconstruction protocol (highres) integrated into the Scipion software.
  • To demonstrate the superiority of the highres method over standard projection matching techniques.

Main Methods:

  • Development of the highres protocol within the Scipion platform.
  • Detailed description of image angular alignment and map reconstruction steps.
  • Modification of the standard projection matching algorithm, focusing on significant feature detection.

Main Results:

  • The highres protocol successfully produced higher resolution maps compared to existing methods.
  • Resolution was validated using Fourier Shell Correlation (FSC), Monogenic Local Resolution, and EMRinger metrics.
  • Enhanced detection of significant features within the reconstructed cryo-EM volumes.

Conclusions:

  • The new highres protocol represents a significant advancement in cryo-EM map reconstruction.
  • This method enables the generation of higher resolution structural data, advancing biological insights.
  • The integration into Scipion ensures accessibility for the research community.