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Updated: Feb 6, 2026

Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS
Published on: February 24, 2021
Recent development and trends in sample extraction and preparation for mass spectrometric analysis of nucleotides,
Tadashi Hayama1, Kaname Ohyama2
1Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, Johnan, Fukuoka 814-0180, Japan.
Abstract:
This review describes the recent developments in sample extraction and preparation techniques for mass spectrometric analysis of nucleotides, nucleosides, and proteins. Unique materials and techniques have been developed for highly selective extraction of nucleotides and nucleosides by solid-phase extraction strategies using various affinities. However, for proteins, the analysis of small-scale sections of diseased tissues (formalin-fixed, paraffin-embedded tissues) and the direct analysis of an exact lesion on the surface of diseased tissues (liquid extraction surface analysis) have become important advances in this field. In this review, we focus on the latest developments of these techniques and strategies.
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