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Updated: Feb 6, 2026

Chromatin Immunoprecipitation ChIP of Histone Modifications from Saccharomyces cerevisiae
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Effects of telomerase inhibitor on epigenetic chromatin modification enzymes in malignancies

Cigir Biray Avci1, Fatma Dogan1, Neslihan Pinar Ozates Ay1

  • 1Department of Medical Biology, Ege University Medical School, Izmir, Turkey.

Journal of Cellular Biochemistry
|August 27, 2018
PubMed

Insights

The telomerase inhibitor BIBR1532 shows significant epigenetic effects in both hematologic malignancies and solid tumors. This compound effectively induced apoptosis in glioblastoma cells and regulated key epigenetic genes in leukemia and glioblastoma cell lines.

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • Telomerase is crucial for cell proliferation, tumor growth, and therapy resistance.
  • Telomerase inhibitors, like BIBR1532, target telomerase components TERC and TERT.
  • Understanding the epigenetic impact of telomerase inhibition is vital for cancer therapy.

Purpose of the Study:

  • To investigate the epigenetic effects of the telomerase inhibitor BIBR1532.
  • To assess BIBR1532's impact on hematologic malignancies and solid tumors.
  • To compare epigenetic changes in K-562 (leukemia) and U87MG (glioblastoma) cell lines.

Main Methods:

  • Cytotoxicity assessed using WST-1 assay.
  • Apoptosis detected via annexin V staining.
  • Epigenetic chromatin modification enzyme gene expression analyzed by RNA isolation and sequencing.

Main Results:

  • BIBR1532 induced a 2.41-fold increase in apoptosis in U87MG glioblastoma cells.
  • Apoptosis was also observed, though to a lesser extent, in K-562 leukemia cells.
  • BIBR1532 demonstrated regulation of similar epigenetic genes in both tested cell lines.

Conclusions:

  • Telomerase inhibitor BIBR1532 exhibits significant epigenetic effects on both hematologic malignancies and solid tumors.
  • BIBR1532's ability to modulate epigenetic mechanisms suggests therapeutic potential.
  • The study highlights BIBR1532 as a promising agent for targeting cancer through epigenetic pathways.
Keywords:
BIBR1532chronic myeloid leukemiaglioma

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