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Moloney murine sarcoma virus encoded p37mos expressed in yeast has protein kinase activity

B Singh, C Wittenberg, S I Reed

    Virology
    |July 30, 1986
    PubMed

    Insights

    The Moloney murine sarcoma virus env-mos protein (p37mos) was successfully expressed in yeast. The yeast-produced p37mos protein kinase demonstrated autophosphorylation activity, consistent with findings in mouse cells.

    Area of Science:

    • Molecular Biology
    • Virology
    • Yeast Genetics

    Background:

    • The Moloney murine sarcoma virus env-mos protein (p37mos) is a known protein kinase.
    • Previous studies characterized p37mos activity in virus-infected mouse cells.

    Purpose of the Study:

    • To express the Moloney murine sarcoma virus env-mos protein (p37mos) in yeast.
    • To characterize the biochemical activity of yeast-produced p37mos.

    Main Methods:

    • Gene expression in yeast using the GAL1 promoter.
    • Immune complex kinase assay using anti-mos (37-35) serum.

    Main Results:

    • Successful expression of p37mos in yeast.
    • Yeast-produced p37mos exhibited autophosphorylation activity.
    • Activity of yeast-derived p37mos was consistent with that from mouse cells.

    Conclusions:

    • Yeast is a viable system for producing functional p37mos protein.
    • The autophosphorylation activity of p37mos is conserved in yeast expression systems.

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