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Detection and characterization of a clinical Escherichia coli ST3204 strain coproducing NDM-16 and MCR-1

Xi Li1, Xinli Mu2, Ping Zhang2

  • 1Centre of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical college, Hangzhou, Zhejiang 310014, China, forgood2000@163.com.

Infection and Drug Resistance
|August 28, 2018
PubMed
Abstract

Insights

This study reports the first isolation of an extensively drug-resistant Escherichia coli strain co-producing NDM-16 and MCR-1. This finding highlights the urgent need for surveillance to prevent the spread of these dangerous resistance genes.

Area of Science:

  • Microbiology
  • Genetics
  • Antimicrobial Resistance

Background:

  • The plasmid-mediated colistin resistance gene, mcr-1, is a global health concern.
  • Spread of mcr-1 into carbapenem-resistant Enterobacteriaceae (CRE) leads to extensively drug-resistant (XDR) and pan drug-resistant (PDR) isolates.
  • These resistant isolates pose a significant therapeutic challenge, potentially causing untreatable infections.

Purpose of the Study:

  • To report the isolation and characterization of an extensively drug-resistant Escherichia coli strain co-producing NDM-16 and MCR-1.
  • To analyze the genetic basis and plasmid characteristics of this multi-drug resistant isolate.
  • To emphasize the clinical significance of co-occurring transferable resistance genes.

Main Methods:

  • Whole-genome sequencing of the E. coli strain EC1188.
  • Genotyping, antimicrobial susceptibility testing, and conjugation experiments.
  • Plasmid analysis using S1-pulsed-field gel electrophoresis and southern blot hybridization.
  • Comparative genetic analysis of resistance-harboring plasmids.

Main Results:

  • Isolation of XDR E. coli strain EC1188 from a patient's urine sample.
  • Identification of co-produced NDM-16 and MCR-1 resistance genes.
  • NDM-16 and MCR-1 were located on distinct plasmids (~65 kb and ~80 kb, respectively).
  • Successful transfer of resistance phenotype to E. coli C600 via conjugation.
  • Plasmids showed high similarity to previously reported NDM-5 and MCR-1 carrying plasmids.

Conclusions:

  • This is the first report of an E. coli strain co-producing NDM-16 and MCR-1.
  • The blaNDM-16-harboring plasmid was characterized for the first time.
  • The co-occurrence of these prevalent transferable resistance genes in a single isolate is highly significant.
  • Active surveillance is imperative to prevent further dissemination of MCR-1-producing CRE isolates.
Keywords:
CREE. coliNDM-16colistin resistanceextensively drug-resistant bacteriamcr-1

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