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Label-Retention Expansion Microscopy LR-ExM Enables Super-Resolution Imaging and High-Efficiency Labeling
Published on: October 11, 2022
Bifunctional Super-resolution Imaging Probe with Acidity-Independent Lysosome-Retention Mechanism
Zhongwei Xue1, Siyu Wang1, Jian Li1
1Department of Chemical Biology, College of Chemistry and Chemical Engineering, State Key Laboratory for Physical Chemistry of Solid Surfaces, the Key Laboratory for Chemical Biology of Fujian Province, The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, and Innovation Center for Cell Signaling Network , Xiamen University , Xiamen , 361005 , China.
Abstract:
Spatiotemporal imaging is of enormous use to explore organelle biology, necessitating organelle-tracing techniques reliable in varied cell stress. We herein reported lysosomal imaging using rhodamine-X-integrated sialic acid (ROXSA), which is stably maintained in lysosomes irrespective of lysosomal pH changes. Exhibiting bright fluorescence and superior photostability, ROXSA enables 120 h continual tracking of fusion/fission of lysosomes and mitochondrion-lysosome interaction in mitophagy. Relative to conventional acidotropic probes prone to dissipation from stressed lysosomes, ROXSA offers a new route for long-term tracking of stressed lysosomes relevant to diverse pathological conditions.
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