Related Experiment Video
Updated: Feb 6, 2026

Author Spotlight: An Antimicrobial Fabric Using Nano-Herbal Encapsulation of Essential Oils
Published on: April 7, 2023
Stability and antimicrobial activity of eucalyptus essential oil emulsions
Angélica Clavijo-Romero1, María X Quintanilla-Carvajal2, Yolanda Ruiz2
11 Maestría en Diseño y Gestión de Procesos, Universidad de La Sabana, Chía, Colombia.
Abstract:
We evaluated various formulations of oil-in-water emulsions prepared from eucalyptus essential oil, for their stability and antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. These formulations were developed using a response surface experimental design and analyzed with Design-Expert® 10 software. The emulsions were prepared in a colloid mill, and emulsion characterization was performed using the zeta ( ζ)-potential, droplet size distribution, and phase separation. The antimicrobial effects were assessed by death kinetics. The droplet size and ζ-potential of the 16 emulsions ranged from 1.071 to 1.865 µm (based on Feret's diameter) and -34.8 to -24 mV, respectively. Three formulations (14, 15, and 16) demonstrated the highest stability parameters (no phase separation) during the 28 days of evaluation. Eucalyptus essential oil emulsions exhibited antimicrobial activity against E. coli, S. aureus, and P. aeruginosa in less than 1 min.
Related Concept Videos
RNA Stability
Antimicrobial Effectiveness
Compounds Essential to Human Function
Inorganic Compounds Essential to Human Functioning
Inorganic compounds essential to human functioning include water, salts, acids, and bases. These compounds are inorganic, i.e., they do not have a carbon-hydrogen bond. Water...
Essential Minerals for Bone Health
Calcium and Phosphorus
Calcium is a critical component of bones, especially in the form of calcium phosphate and calcium carbonate. Since the body cannot make calcium, it must be obtained from the diet. However, calcium cannot be absorbed from the small intestine without...
Nuclear Stability
To hold positively charged protons together...
Antimicrobial Proteins
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...

