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Updated: Aug 15, 2026

Generation of Composite Plants in Medicago truncatula used for Nodulation Assays
Published on: March 27, 2011
Mobilization of Tn5 insertions from Rhizobium fredii by pJB3JI
Abstract:
Techniques for in vivo cloning were used with the fast-growing nitrogen-fixing soybean microsymbiont R. fredii USDA 191. Selection for transfer of Tn5 insertions from R. fredii USDA 191 containing the gene-mobilizing plasmid pJB3JI provided recombinants at up to 400 times the background mutation level. These techniques may be useful for future genetic analysis of R. fredii.
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