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Related Experiment Video

Updated: Feb 6, 2026

Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles

Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles

Published on: September 4, 2019

INTACT Proteomics in Xenopus.

Lauren Wasson^1,2, Nirav M Amin^2,3, Frank L Conlon^4,2,3

¹Department of Genetics, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina 27599.

Cold Spring Harbor Protocols

|August 29, 2018

Summary

This summary is machine-generated.

Researchers developed a method to isolate pure cell populations from tissues using INTACT (isolation of nuclei tagged in specific cell types). This allows for detailed proteomics analysis of nuclear protein complexes for better understanding of cellular processes.

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Skeletal Muscle Gender Dimorphism from Proteomics

Skeletal Muscle Gender Dimorphism from Proteomics

Published on: December 14, 2011

Area of Science:

Molecular Biology
Cell Biology
Proteomics

Background:

Analyzing molecular mechanisms in heterogeneous tissues is challenging.
Isolating pure cell populations is crucial for accurate cell-type specific functional assessment.
Understanding nuclear protein complexes requires pure cell samples.

Purpose of the Study:

To describe a protocol for isolating pure cell populations from Xenopus.
To enable proteomics analysis of nuclear protein complexes from specific cell types.
To adapt the INTACT method for diverse tissue proteomics studies.

Main Methods:

Utilized the INTACT (isolation of nuclei tagged in specific cell types) method in Xenopus.
Employed two transgenes: a nuclear targeting fusion (NTF) and biotin ligase BirA.
Drove transgenes with tissue-specific promoters for targeted nuclear isolation.

Main Results:

Successfully isolated nuclei from specific cell types using the INTACT protocol.
Enabled visualization of nuclear targeting fusion (NTF) via EGFP and BirA via mCherry.
Facilitated proteomics analysis of nuclear protein complexes from purified cell populations.

Conclusions:

The INTACT method provides a robust approach for isolating pure cell nuclei.
This technique is adaptable for proteomics studies across various Xenopus tissues.
Facilitates determination of protein complex composition in specific cell types.