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Effect of Radiotherapy on Cariogenic Organism Streptococcus sobrinus in Saliva in Head and Neck Cancer: A Clinical
Sonali Kapoor1, Shilpi R Daveshwar2, Kesha Sheth2
1Department of Conservative Dentistry, Esthetics and Endodontics, Manubhai Patel Dental College and Hospital Vadodara, Gujarat, India, Phone: +919825323057,
Aim:
Aim of the study was to assess salivary Streptococcus sobrinus in head and neck cancer using quantitative polymerase chain reaction (PCR).
Materials And Methods:
Unstimulated saliva samples were collected from head and neck cancer patient preradiotherapy. Unstimulated saliva samples were collected from oral and laryngeal cancer patients after 6 weeks of radiotherapy (dose 60 Gy). The subjects were explained not to consume solids or liquids or carry out any dental hygiene activity 1 hour prior to saliva collection. Accumulated unstimulated saliva was collected in cylindrical tube through funnel. The collected saliva was then transferred to Eppendorf tube containing Tris-ethylenediamine-tetraacetic acid (EDTA) (TE) buffer and was transported to lab for real-time PCR analysis.
Results:
Streptococcus sobrinus significantly increased post-radiotherapy as compared with preradiotherapy in head and neck cancer patients.
Conclusion:
Within the limitation of this study, we conclude that amount of S. sobrinus increases postradiotherapy in head and neck cancer patients.
Clinical Significance:
As radiation therapy has harmful effects on hard and soft tissues of oral cavity, dentists should provide motivation for oral health care to the patients.
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