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Related Experiment Video

Updated: Feb 6, 2026

Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds

Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds

Published on: October 29, 2015

Deciphering Virus Entry with Fluorescently Labeled Viral Particles.

Anja B Hoffmann¹, Magalie Mazelier¹, Psylvia Léger¹

¹From CellNetworks Cluster of Excellence and Department of Infectious Diseases, Virology, University Hospital Heidelberg, Heidelberg, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|August 29, 2018

Summary

This summary is machine-generated.

Fluorescently labeling viral particles enables detailed study of virus entry into host cells. This technique, combined with advanced microscopy, visualizes and quantifies each step of viral infection.

Keywords:

Endocytosis Flow cytometry Fluorescent dyes Fluorescently labeled viral particles Fluorimetry Intracellular trafficking Membrane fusion Microscopy Single viral particle tracking Virus entry

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Last Updated: Feb 6, 2026

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Method for Measurement of Viral Fusion Kinetics at the Single Particle Level

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level

Published on: September 7, 2009

Area of Science:

Virology
Cell Biology
Biophysics

Background:

Viral infection requires entry into host cells, a complex process involving attachment, receptor binding, and internalization.
Investigating these dynamic events has been challenging due to their intricate nature.
Advancements in fluorescence technologies have revolutionized the study of viral entry.

Purpose of the Study:

To describe a procedure for fluorescently labeling viral particles.
To demonstrate how this labeling can be used to investigate virus entry mechanisms.
To highlight the application of modern fluorescence techniques in virology research.

Main Methods:

Fluorescent labeling of viral particles.
High-speed confocal microscopy.
Total internal reflection microscopy.
Flow cytometry.
Fluorimetry.

Main Results:

Successful visualization of single viral particles in fixed and living cells.
Quantification of individual steps in the virus entry pathway.
Demonstration of fluorescence-based techniques for deciphering viral entry dynamics.

Conclusions:

Fluorescent labeling is a powerful tool for studying viral entry.
Advanced fluorescence microscopy and cytometry provide unprecedented insights into virus-host interactions.
These methods facilitate a deeper understanding of the molecular mechanisms governing viral infection.