Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Electron Microscopy01:25

Overview of Electron Microscopy

14.8K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
14.8K
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

5.5K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
5.5K
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

7.3K
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
7.3K
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

5.5K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
5.5K
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

4.4K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
4.4K
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

7.2K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
7.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diatom ultrastructural diversity across controlled and natural environments.

Current biology : CB·2025
Same author

Crosshair, semi-automated targeting for electron microscopy with a motorised ultramicrotome.

eLife·2022
Same author

Integrative Imaging Reveals SARS-CoV-2-Induced Reshaping of Subcellular Morphologies.

Cell host & microbe·2020
Same author

Spatiotemporal Coupling of the Hepatitis C Virus Replication Cycle by Creating a Lipid Droplet- Proximal Membranous Replication Compartment.

Cell reports·2019
Same author

Lipid droplet quantification based on iterative image processing.

Journal of lipid research·2019
Same author

An alternative membrane topology permits lipid droplet localization of peroxisomal fatty acyl-CoA reductase 1.

Journal of cell science·2019

Related Experiment Video

Updated: Feb 6, 2026

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
11:16

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Published on: August 7, 2016

10.2K

3D Electron Microscopy (EM) and Correlative Light Electron Microscopy (CLEM) Methods to Study Virus-Host

Inés Romero-Brey1

  • 1Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Heidelberg, Germany. ines_romero-brey@med.uni-heidelberg.de.

Methods in Molecular Biology (Clifton, N.J.)
|August 29, 2018
PubMed
Summary

Electron microscopy (EM) visualizes virus-host interactions in 3D. Correlative light electron microscopy (CLEM) integrates light microscopy (LM) to pinpoint infected cells for detailed ultrastructural analysis.

Keywords:
Chemical fixationCorrelative light and electron microscopyCryo-immobilizationElectron tomographyFocused ion beam-scanning electron microscopyFreeze-substitutionHigh-pressure freezingUltrastructureVirus-induced cellular rearrangementsVirus-infected cells

More Related Videos

Correlative Light Electron Microscopy CLEM for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells
09:18

Correlative Light Electron Microscopy CLEM for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells

Published on: September 7, 2018

81.8K
Correlative Light and Electron Microscopy CLEM as a Tool to Visualize Microinjected Molecules and their Eukaryotic Sub-cellular Targets
09:10

Correlative Light and Electron Microscopy CLEM as a Tool to Visualize Microinjected Molecules and their Eukaryotic Sub-cellular Targets

Published on: May 4, 2012

19.7K

Related Experiment Videos

Last Updated: Feb 6, 2026

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
11:16

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Published on: August 7, 2016

10.2K
Correlative Light Electron Microscopy CLEM for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells
09:18

Correlative Light Electron Microscopy CLEM for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells

Published on: September 7, 2018

81.8K
Correlative Light and Electron Microscopy CLEM as a Tool to Visualize Microinjected Molecules and their Eukaryotic Sub-cellular Targets
09:10

Correlative Light and Electron Microscopy CLEM as a Tool to Visualize Microinjected Molecules and their Eukaryotic Sub-cellular Targets

Published on: May 4, 2012

19.7K

Area of Science:

  • Virology
  • Cell Biology
  • Microscopy

Background:

  • Viruses employ diverse strategies for host interaction, infection, and propagation.
  • High-resolution visualization techniques are crucial for understanding virus-host dynamics.

Purpose of the Study:

  • To describe electron microscopy (EM) methods for 3D ultrastructural analysis of virus-infected cells.
  • To explain correlative light electron microscopy (CLEM) for precise localization of cellular events.

Main Methods:

  • 3D ultrastructural analysis using electron microscopy (EM).
  • Integration of light microscopy (LM) for cell allocation.
  • Sample preparation protocols for correlative light electron microscopy (CLEM).

Main Results:

  • EM methods enable detailed visualization of virus-infected cell ultrastructure.
  • CLEM facilitates the identification of specific cellular phenotypes induced by viral proteins.
  • Applicability of described methods to various cultured cells, including influenza-infected cells.

Conclusions:

  • EM and CLEM are powerful tools for studying virus-host interactions at the ultrastructural level.
  • These techniques enhance the understanding of viral infection mechanisms and cellular responses.
  • The described methods support research in virology and cell biology.