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Erythrocyte Sedimentation Rate: A Physics-Driven Characterization in a Medical Context
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Differences in erythrocyte sedimentation rates using a modified Westergren method and an alternate method.

Elise Schapkaitz1, Shilla RabuRabu2, Marcel Engelbrecht1

  • 1Department of Molecular Medicine and Hematology, Faculty of Health Sciences, University of Witwatersrand Medical School, Johannesburg, South Africa.

Journal of Clinical Laboratory Analysis
|August 29, 2018
PubMed
Summary
This summary is machine-generated.

The iSED photometric aggregometry method for erythrocyte sedimentation rate (ESR) showed acceptable performance but differed from the Westergren method, especially at high ESR values. Further monitoring of novel ESR methods is recommended.

Keywords:
EDTA tubealternate erythrocyte sedimentation rate measurementerythrocyte sedimentation rateiSEDverification

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Area of Science:

  • Clinical pathology
  • Hematology
  • Diagnostic methods

Background:

  • Laboratories globally utilize modified methods for erythrocyte sedimentation rate (ESR) measurements.
  • The iSED, a novel photometric aggregometry method, offers enhanced operator safety and reduced analysis time.
  • This study assesses the iSED's diagnostic utility in a South African population with inflammatory conditions.

Purpose of the Study:

  • To evaluate the diagnostic performance of the novel iSED ESR method.
  • To compare iSED results with the established modified Westergren method.
  • To assess the iSED's utility in a diverse South African patient cohort.

Main Methods:

  • Comparison of iSED with the predicate modified Westergren method (StaRRsed) using K2 EDTA samples.
  • Analysis across three ESR ranges (<20, 20-80, >80 mm/h) in 120 pediatric and adult patients.
  • Evaluation of precision, stability, and carryover according to International Council for Standardisation in Haematology guidelines.

Main Results:

  • iSED demonstrated acceptable imprecision and minimal carryover (2.86%).
  • Correlation coefficients varied across ESR ranges (r=0.58-0.71).
  • A significant mean difference of 7.99 mm/h was observed between iSED and Westergren methods (P < 0.001), particularly at extremes.

Conclusions:

  • The iSED method shows differences in ESR results compared to the Westergren method, notably at the analytical extremes.
  • Stability of iSED measurements was confirmed up to 24 hours under various storage conditions.
  • Careful consideration and ongoing performance monitoring of novel ESR methods like iSED are advised.