Related Experiment Video
Updated: Feb 6, 2026

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
Published on: August 20, 2018
Associative and Dissociative Processes in Non-Covalent Polymer-Mediated Intracellular Protein Delivery
Nicholas D Posey1, Gregory N Tew1,2,3
1Department of Polymer Science and Engineering, University of Massachusetts Amherst, Amherst, MA, 01003, USA.
Abstract:
Functional protein delivery has created new opportunities for studying intracellular processes and discovering new therapeutics. To that end, researchers have pursued intracellular protein delivery by using an increasing number of methods. This focus review will highlight polymeric carriers that non-covalently bind and deliver protein cargo in vitro. The correlation between polymer-protein binding and delivery as well as the correlation between complex-membrane binding and delivery is reviewed. Finally, binding and its relation to the intracellular function of the protein post-delivery is considered. The purpose of this review is to evaluate the role that binding interactions play in the non-covalent protein-delivery landscape. Presently, the literature does not adequately resolve how binding throughout the process ultimately affects delivery. The field does contain preliminary insights that are expected to impact future delivery applications when developed further.
Related Concept Videos
Covalently Linked Protein Regulators
These groups modify specific amino acids in a protein....
Covalently Linked Protein Regulators
Polymers
Covalent Bonds
Covalent Bonds
When two atoms share electrons to complete their valence shells, they create a covalent bond. An atom's electronegativity—the force with which shared electrons are pulled towards an atom—determines how the electrons are shared. Molecules formed with covalent bonds can be either polar or nonpolar. Atoms with similar electronegativities form nonpolar covalent bonds; the electrons are shared equally. Atoms with different electronegativities share electrons unequally,...
Network Covalent Solids
To break or to melt a covalent network solid, covalent bonds must be broken. Because covalent bonds are relatively strong, covalent network solids are typically...

