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Reverse Transcription Loop-Mediated Isothermal Amplification RT-LAMP Assay for the Specific and Rapid Detection of Tilapia Lake Virus
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LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP): SENSITIVE AND RAPID DETECTION OF SCHISTOSOMA HAEMATOBIUM DNA IN URINE
Journal of the Egyptian Society of Parasitology
|August 29, 2018
Summary
Loop-mediated isothermal DNA amplification (LAMP) offers a sensitive and simple diagnostic tool for detecting Schistosoma haematobium DNA. This technique shows promise for diagnosing urogenital schistosomiasis in resource-limited settings.
Area of Science:
- Medical Parasitology
- Infectious Diseases
- Molecular Diagnostics
Background:
- Schistosomiasis haematobium is a prevalent parasitic disease in tropical regions, causing significant urinary tract pathology and potentially leading to bladder cancer.
- Current diagnostic methods like microscopy have limitations in sensitivity and reliability, especially in chronic cases.
- Advanced techniques such as PCR are often inaccessible in low-income endemic areas due to cost and equipment requirements.
Purpose of the Study:
- To evaluate the diagnostic performance of loop-mediated isothermal DNA amplification (LAMP) for detecting Schistosoma haematobium DNA in urine samples.
- To compare the efficacy of LAMP with the conventional microscopy method for diagnosing urogenital schistosomiasis.
- To assess the potential of LAMP as a cost-effective and sensitive diagnostic tool for resource-limited settings.
Main Methods:
- Urine samples from suspected urogenital schistosomiasis patients were tested using both LAMP and microscopy.
- The specificity of LAMP was confirmed by testing against DNA from other parasites.
- Diagnostic parameters including sensitivity, specificity, and likelihood ratios were calculated for the LAMP assay.
Main Results:
- LAMP demonstrated 100% sensitivity and 63.16% specificity in detecting Schistosoma haematobium DNA.
- The assay showed no cross-reactivity with DNA from other parasitic species.
- A negative likelihood ratio of 0.0 indicates LAMP's high reliability in ruling out infection when negative.
Conclusions:
- Loop-mediated isothermal DNA amplification (LAMP) is a highly sensitive and specific diagnostic tool for urogenital schistosomiasis.
- LAMP is a simple, valuable technique suitable for clinical diagnosis and disease surveillance in resource-limited areas.
- This study represents the first national evaluation of LAMP for diagnosing urogenital schistosomiasis, highlighting its potential impact.
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