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Updated: Feb 6, 2026

Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models
Published on: October 22, 2012
Optical molecular imaging of lysyl oxidase activity - detection of active fibrogenesis in human lung tissue
Tashfeen Aslam1, Amy Miele2, Sunay V Chankeshwara1
1School of Chemistry , EaStChem , University of Edinburgh , Joseph Black Building, West Mains Road , Edinburgh , EH9 3FJ , UK .
Abstract:
Aberrant fibrogenesis is a feature of many diseases in multiple organ systems. The lysyl oxidase family of enzymes are central to tissue homeostasis and elevated lysyl oxidase activity is implicated in fibroproliferation as well as in cancer stroma. We have synthesised a novel fluorogenic reporter for monitoring lysyl oxidase activity that generates a 3-5 fold increase in fluorescence following probe activation in ventilating fibrotic ex vivo asinine lung and ex vivo human lung tissue. The probe termed "oLOX" can provide real-time measurement of lysyl oxidase activity in a number of biological settings and is tractable from an in vitro setting to man.
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