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Related Concept Videos

Adhesion01:14

Adhesion

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Adhesion occurs when one type of molecule is attracted to a different molecule. Water exhibits adhesive properties in the presence of polar surfaces, such as glass or cellulose in plants. For instance, when water is poured into a glass, the positively charged hydrogen molecules of water are more attracted to the negatively charged oxygen molecules in the silica than to the oxygen in neighboring water molecules.
Capillary action is a result of water’s adhesive tendencies. When a narrow...
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Cell Adhesion in Plants01:14

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Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses.
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Immunoglobulin-like Cell Adhesion Molecules01:31

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Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
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Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Cell Adhesion Molecules - Types and Functions01:20

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Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily  involved...
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Intracellular Signaling Affects Focal Adhesions01:17

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Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
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Related Experiment Video

Updated: Feb 6, 2026

Quantitation of Endothelial Cell Adhesiveness In Vitro
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Quantitative Proteomics Implicates Rictor/mTORC2 in Cell Adhesion.

Hao Wang1, Xianfeng Shao2, Qian He3

  • 1Department of Genetics, School of Basic Medical Sciences , Tianjin Medical University , Tianjin 300070 , P.R. China.

Journal of Proteome Research
|August 30, 2018
PubMed
Summary
This summary is machine-generated.

Mammalian target of rapamycin complex 2 (mTORC2) regulates cell adhesion and invasion. Reducing Rictor, an mTORC2 component, alters proteome, impacting cell adhesion molecules and promoting renal cancer cell invasion.

Keywords:
AktRictorTMT labelingcell adhesioncell migrationgene expressionmTORC2mass spectrometryquantitative proteomicsrenal cancer

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Area of Science:

  • Cellular Biology
  • Molecular Oncology
  • Proteomics

Background:

  • Mammalian target of rapamycin complex 2 (mTORC2) is crucial for cellular functions.
  • Rictor is a specific component of mTORC2, essential for its activity.

Purpose of the Study:

  • To investigate the proteomic changes and functional roles of mTORC2 by reducing Rictor expression.
  • To elucidate the molecular mechanisms by which mTORC2 influences renal cancer cell behavior.

Main Methods:

  • Established a stable Rictor knockdown cell line.
  • Performed quantitative proteomic analysis to identify differentially expressed proteins.
  • Constructed a protein-protein interaction network.
  • Utilized gene ontology analysis for functional enrichment.
  • Investigated the role of AKT signaling pathway.

Main Results:

  • Identified 101 downregulated and 50 upregulated proteins in Rictor knockdown cells.
  • Established a Rictor/mTORC2-regulated protein-protein interaction network.
  • Demonstrated significant involvement of Rictor/mTORC2 in cell adhesion processes.
  • Observed altered expression of key cell adhesion molecules, including ITGA5, TGFB1I1, and LOXL2.
  • Showed that Rictor/mTORC2 modulates cell adhesion and invasion via AKT signaling.

Conclusions:

  • Rictor/mTORC2 regulates a significant portion of the cellular proteome.
  • Rictor/mTORC2 plays a critical role in cell adhesion and renal cancer cell invasion.
  • Modulation of cell adhesion molecules by Rictor/mTORC2, potentially through AKT, drives cancer cell migration and invasion.