Quantitative determination of fenclorac in serum

Area of Science:

• Analytical Chemistry
• Pharmacokinetics

Background:

• Fenclorac analysis in biological fluids is crucial for pharmacokinetic and clinical studies.
• Existing methods may lack specificity or require complex procedures.

Purpose of the Study:

• To develop and validate a spectrophotometric method for quantifying fenclorac and its primary metabolite in human serum.
• To assess the method's accuracy and correlation with established techniques.

Main Methods:

• Serum samples undergo extraction to isolate fenclorac and its metabolite.
• Alkaline conversion transforms fenclorac to its alpha-hydroxy acid metabolite.
• Oxidation yields a benzaldehyde derivative, measured spectrophotometrically at 252 nm.

Main Results:

• The developed method quantifies fenclorac (≥90%) and its metabolite (~10%) in serum.
• A high correlation (90%) was observed between the spectrophotometric method and 14C-data.
• The method is effective over a concentration range of 1.4-25.5 µg/ml.

Conclusions:

• A reliable and efficient spectrophotometric assay for fenclorac and its metabolite in human serum has been established.
• This method provides accurate concentration data, supporting clinical and research applications.