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Updated: Feb 6, 2026

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples
Published on: July 28, 2016
Selective Monitoring and Imaging of Eosinophil Peroxidase Activity with a J-Aggregating Probe
Tae-Il Kim1, Byunghee Hwang1, Boeun Lee2
1Department of Chemistry and Research Institute of Basic Sciences , Kyung Hee University , 26 Kyungheedae-ro , Dongdaemun-gu, Seoul 02447 , Korea.
Abstract:
The specific detection of eosinophil peroxidase (EPO) activity requires the difficult distinction between hypobromous acid generated by EPO and hypochlorous acid generated by other haloperoxidases. Here we report a fluorogenic probe that is halogenated with high kinetic selectivity (≥1200:1) for HOBr over HOCl. Heavy-atom effects do not quench the dibrominated product because of its self-assembly into emissive J-aggregates that provide a turn-on signal. Applications of this fluorogen to EPO activity assays, dipstick sensors, fluorescence imaging of EPO activity, assays of oxidative stress in cancer cells, and immune response detection in live mice are reported.
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