Related Experiment Video
Updated: Feb 6, 2026

Anticancer Efficacy of Photodynamic Therapy with Lung Cancer-Targeted Nanoparticles
Published on: December 1, 2016
Two-Photon Excited FRET Dyads for Lysosome-Targeted Imaging and Photodynamic Therapy
Mengliang Zhu1, Jinghui Zhang1, Yabin Zhou2
1Beijing Key Laboratory for Science and Application of Functional Molecular and Crystalline Materials, Department of Chemistry , University of Science and Technology Beijing , Beijing 100083 , China.
Abstract:
Two-photon excitable fluorescent dyes with integrated functions of targeted imaging and photodynamic therapy (PDT) are highly desired for the development of cancer theranostic agents. Herein, fluorescence resonance energy transfer (FRET) dyads, AceDAN-H2Por-Lyso (1a) and AceDAN-ZnPor-Lyso (1b), were developed for two-photon excited (TPE) lysosome-targeted fluorescence imaging and PDT of cancer cells. Under one-photon or two-photon excitation, the AceDAN donor can effectively transfer the excited state energy to the porphyrin acceptor via high efficient FRET, leading to the generation of deep-red fluorescence and singlet oxygen for cell imaging and PDT, respectively. 1a and 1b exhibit high photocytotoxicity and low dark cytotoxicity, in addition to strong lysosomal targeting capability in living cells. By taking the advantages of the two-photon absorption properties of the AceDAN donor and the properly distributed S1 and T1 states of the porphyrin acceptor, the AceDAN-porphyrin dyads 1a and 1b have been successfully applied to TPE-fluorescence imaging for tracking the significant morphology changes of cancer cells under two-photon laser irradiation.
Related Concept Videos
Targeted Cancer Therapies
There are several types of targeted therapies against...
Lysosomes
Lysosomal Hydrolases
Delivery Pathways to the Lysosome
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Gene Therapy
Excitation-Contraction Coupling in Skeletal Muscles
When an action...

