Related Experiment Video
Updated: Feb 6, 2026
10:42
Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models
Published on: October 22, 2012
16.8K
NADPH-oxidase-derived ROS alters cell migration by modulating adhesions dynamics
Maurício Tavares Tamborindeguy1,2, Bibiana Franzen Matte1, Grasieli de Oliveira Ramos1,3
1Basic Research Center in Dentistry, Dentistry School, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Biology of the Cell
|August 30, 2018
Summary
Reactive oxygen species (ROS) generated by NADPH oxidase are crucial for cell migration. Inhibiting ROS significantly reduces migration speed and alters adhesion dynamics, highlighting ROS as a potential therapeutic target.
Area of Science:
- Cell Biology
- Biochemistry
- Molecular Biology
Background:
- Cell migration is a fundamental biological process regulated by complex molecular signaling pathways.
- Rho GTPase Rac1 plays a key role in cell migration and NADPH oxidase assembly.
- NADPH oxidase generates reactive oxygen species (ROS), suggesting a role for ROS in cell migration.
Purpose of the Study:
- To investigate the specific effects of NADPH oxidase-derived ROS on cell migration dynamics.
- To elucidate the molecular mechanisms by which ROS influences cell migration processes.
Main Methods:
- Utilized time-lapse microscopy to observe cell migration in CHO.K1 cells.
- Employed N-acetyl-cysteine (NAC) and diphenyliodonium (DPI) to modulate ROS levels.
- Analyzed protrusion dynamics, adhesion processes, and signaling pathways using pull-down assays and TIRF microscopy.
Main Results:
- ROS depletion via NAC or DPI significantly decreased cell migration speed by approximately 50% and impaired directionality.
- NADPH oxidase inhibition led to increased protrusion rates, but these were less stable.
- ROS modulation affected downstream signaling, reducing FAK-Y397 levels and increasing cell adhesion size.
Conclusions:
- NADPH oxidase-derived ROS are essential modulators of cell migration.
- ROS influences cell migration primarily through alterations in adhesion dynamics and associated signaling pathways.
- ROS represents a potential therapeutic target for diseases involving aberrant cell migration.
Related Concept Videos
01:19Cell Migration
6.6K
Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
6.6K
01:09Cell Migration
18.8K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
18.8K
01:14Adhesion
44.5K
Adhesion occurs when one type of molecule is attracted to a different molecule. Water exhibits adhesive properties in the presence of polar surfaces, such as glass or cellulose in plants. For instance, when water is poured into a glass, the positively charged hydrogen molecules of water are more attracted to the negatively charged oxygen molecules in the silica than to the oxygen in neighboring water molecules.
Capillary action is a result of water’s adhesive tendencies. When a narrow...
Capillary action is a result of water’s adhesive tendencies. When a narrow...
44.5K
01:14Cell Adhesion in Plants
3.4K
Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses.
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
3.4K
01:31Immunoglobulin-like Cell Adhesion Molecules
4.4K
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
4.4K
01:30Alterations in Respiration II
1.8K
There are numerous types of normal and abnormal respiration. Based on ventilatory movements, breathing patterns are classified as regular, deep, or shallow. Examples include Biot's breathing, Cheyne-Stokes respiration, Kussmaul's breathing, hyperventilation, and hypoventilation. Each pattern is clinically significant and aids in evaluating patients.
In Biot's breathing, the respiratory rate and depth are irregular, alternating between periods of deep gasping and apnea. Common causes...
In Biot's breathing, the respiratory rate and depth are irregular, alternating between periods of deep gasping and apnea. Common causes...
1.8K