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Diversity of virulence level phenotype of hypervirulent Klebsiella pneumoniae from different sequence type lineage

Qiucheng Shi1,2, Peng Lan3, Danyan Huang1,2

  • 1Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, China.

BMC Microbiology
|August 31, 2018
PubMed
Abstract

Insights

Hypervirulent Klebsiella pneumoniae (hvKP) causes liver abscesses. Multilocus sequence typing (MLST) effectively differentiates hvKP virulence levels, identifying K1 ST23 as prevalent but more common in moderate virulence groups.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Genetics

Background:

  • Hypervirulent Klebsiella pneumoniae (hvKP) is an emerging pathogen causing community-acquired pyogenic liver abscesses, particularly in the Asian-Pacific region.
  • The emergence of multidrug-resistant hvKP (MDR-hvKP) in Europe and Asia highlights the need for clear definitions and virulence assessment.
  • International consensus on hvKP definition and virulence level determination remains unclear.

Purpose of the Study:

  • To investigate the characteristics of hvKP isolates.
  • To evaluate methods for assessing hvKP virulence levels.
  • To identify prevalent hvKP clones and their association with virulence.

Main Methods:

  • Collected and analyzed 56 hvKP isolates.
  • Employed string test, capsule serotyping, multilocus sequence typing (MLST), virulence gene detection, and serum resistance assays.
  • Utilized the Galleria mellonella infection model to assess virulence and receiver operating characteristic curves to evaluate assessment methods.

Main Results:

  • 64.3% of isolates exhibited hypermucoviscosity and were K1 or K2 serotypes.
  • K1 serotype isolates were predominantly ST23 (94.4%), while K2 isolates were a mix of ST65, ST86, and ST375.
  • MLST proved to be a reliable method for discriminating hvKP virulence levels, with K1 ST23 being the most prevalent clone, though dominant in the moderate virulence group.

Conclusions:

  • K1 ST23 Klebsiella pneumoniae represents the most common hvKP clone.
  • While prevalent, K1 ST23 is more frequently found in the moderate virulence group.
  • MLST is a reliable method for assessing the virulence of hvKP isolates.
Keywords:
50% lethal doseGalleria. mellonella infection modelHypervirulent K. pneumoniaeMultilocus sequence typingVirulence level

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