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Phorphorylative electron transport chains lacking a cytochrome bc1 complex.

A Kröger, J Paulsen, I Schröder

    Journal of Bioenergetics and Biomembranes
    |June 1, 1986
    PubMed
    Summary
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    Wolinella succinogenes generates less than 1 ATP per 2 electrons using fumarate, with a proton motive force of 0.18 V. Sulfur respiration yields even less ATP and does not involve quinones.

    Area of Science:

    • Microbial metabolism
    • Bioenergetics
    • Anaerobic respiration

    Background:

    • Electron transport-coupled phosphorylation is crucial for ATP synthesis in microorganisms.
    • Fumarate and sulfur serve as terminal electron acceptors in anaerobic respiration.
    • Understanding these pathways provides insights into microbial energy conservation.

    Purpose of the Study:

    • To investigate the bioenergetics of electron transport-coupled phosphorylation in Wolinella succinogenes using fumarate as a terminal electron acceptor.
    • To compare the efficiency of ATP production with fumarate versus sulfur as terminal electron acceptors.
    • To elucidate the components and mechanisms of the electron transport chains involved.

    Main Methods:

    • Analysis of electron transport chains in Wolinella succinogenes and Desulfuromonas acetoxidans.

    Related Experiment Videos

  • Quantification of proton motive force (delta mu H) and H+/electron ratios.
  • Identification of key enzymes such as dehydrogenases and reductases.
  • Investigation of quinone involvement in electron transport.

    • Main Results:

    • Electron transport with fumarate in W. succinogenes yields less than 1 ATP/2 electrons, with a delta mu H of 0.18 V and an H+/electron ratio of 1.
    • The electron transport chain involves hydrogenase, formate dehydrogenase, and fumarate reductase, but not C-type cytochromes.
    • Phosphorylative electron transport using sulfur in W. succinogenes or D. acetoxidans does not involve known quinones, suggesting lower ATP yields.
    • Succinate oxidation by sulfur in D. acetoxidans occurs via reversed electron transport.

    Conclusions:

    • The bioenergetic efficiency of fumarate respiration in W. succinogenes is limited.
    • Sulfur respiration in these bacteria is likely less efficient than fumarate respiration and involves different electron transport components.
    • Reversed electron transport plays a role in specific metabolic pathways like succinate oxidation by sulfur.