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Complete genome analysis of Iranian IS-1494 like avian infectious bronchitis virus

Fatemeh Sadat Mousavi1, Arash Ghalyanchilangeroudi1, Hossein Hosseini2

  • 11Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

Virusdisease
|August 31, 2018
PubMed

Insights

The infectious bronchitis virus (IBV) IS-1494 like strain, prevalent in Iran, shows significant genetic variation. Phylogenetic analysis reveals its complex evolutionary relationships and potential for adaptation, highlighting the role of recombination in IBV evolution.

Area of Science:

  • Veterinary Virology
  • Molecular Evolution
  • Genomics

Background:

  • The nephropathogenic infectious bronchitis virus (IBV) strain IS-1494 like (variant-2; GI-23) is prevalent in Iran.
  • Despite vaccination efforts, this IBV strain continues to circulate and spread internationally.

Purpose of the Study:

  • To determine the full-length genome of the Iranian IS-1494 like IBV (Mahed isolate).
  • To understand the evolutionary relationships and genetic diversity of this IBV strain.

Main Methods:

  • Whole-genome sequencing of the Mahed IBV isolate.
  • Phylogenetic analysis using full-length genome and partial gene sequences (1a, 1b, S1, S2, M, E, N).
  • Sequence homology analysis.

Main Results:

  • The Mahed genome comprises 27,652 nucleotides with mutations in structural genes and thirteen predicted open reading frames (ORFs), including rare ORFs 4b, 4c, and 6b.
  • Phylogenetic analysis placed the Mahed isolate with the QX type strain based on full-length genome data, but with the Q1 strain based on partial 1b and S1 genes.
  • The Mahed isolate shared highest sequence homology with Gray and JMK strains (90.06-90.07%) and least with Vic-s (86.21%).

Conclusions:

  • Evolutionary variation, driven by recombination, is crucial for the adaptation and emergence of new IBV genetic types.
  • The genetic diversity observed in the Iranian IS-1494 like IBV highlights the need for ongoing surveillance and updated control strategies.
Keywords:
Avian infectious bronchitis virusComplete genomeIS-1494 likeIranPhylogenetic study

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