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Rapid Molecular Detection and Differentiation of Influenza Viruses A and B

Rapid Molecular Detection and Differentiation of Influenza Viruses A and B

Published on: January 30, 2017

Molecular detection of ephemeral fever virus among large ruminants in the Philippines

John Emmanuel E Lapira¹, Michelle M Balbin², Lawrence P Belotindos²

¹1College of Veterinary Science and Medicine, Central Luzon State University, 3120 Science City of Muñoz, Nueva Ecija Philippines.

|August 31, 2018

Abstract:

In the Philippines, bovine ephemeral fever (BEF) is currently undetected and considered as an exotic disease of both cattle and water buffaloes. The Philippines until now has no official data regarding the occurrence of BEF. There were no existing control programs or vaccine used for the prevention of the disease. However, there are claims of BEF existence in different water buffalo and cattle farms based on the clinical signs but never confirmed using laboratory test yet. Detection of BEF virus in cattle and water buffalo blood samples was conducted using reverse-transcription PCR targeting the glycoprotein (G) gene, a conserved region in the BEF virus genome. The samples were collected from 22 cattle and 50 water buffaloes with clinical signs suggesting of BEF infection. All water buffalo blood samples were negative while four cattle blood samples turned positive for BEF virus. The G gene partial sequence analysis from two BEF virus positive samples showed close relationship to Australian isolates.

Keywords:

Bovine ephemeral fever Philippines Phylogenetic tree analysis RT-PCR

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