Sphingobacterium praediipecoris sp. nov. isolated from effluent of a dairy manure treatment plant

A novel Gram-reaction-negative, rod-shaped, non-motile bacterium, designated as strain G2-10^T was isolated from effluent of a dairy manure treatment plant. Growth occurred at 20-40 °C (optimum at 25-30 °C), pH 7.0-8.0 (optimum at pH 7.0). The range of NaCl concentration for growth was between 0% and 3% (w/v) (optimum 0-1%, w/v). Comparison of 16S rRNA gene sequence indicated that strain G2-10^T was moderately related to the type strains of Sphingobacterium nematocida M-SX103^T and Sphingobacterium suaedae T47^T with a pair-wise sequence similarity of 94.3% and 94.0%, respectively. The major fatty acid constituents of strain G2-10^T were identified as iso-C_15:0 (37.6%), summed feature 3 (consisting of C_16:1ω7c and/or C_16:1ω6c, 29.6%) and iso-C_17:0 3-OH (15.2%). Phosphatidylethanolamine was the major polar lipids of strain G2-10^T. Sphingophospholipids were present. The isoprenoid quinone was composed of only MK-7. The DNA G + C content of strain G2-10^T was found to be 42.5 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strain G2-10^T represents a novel species within the genus Sphingobacterium, for which the name Sphingobacterium praediipecoris is proposed. The type strain is G2-10^T (= KCTC 52880^T = NBRC 112848^T).