Aujeszky's disease: blocking ELISA for the detection of serum antibodies

A blocking ELISA for the detection of serum antibodies to Aujeszky's disease virus has been developed. The test was designed in particular with a view to examining large numbers of blood samples. It has been found to be sensitive, specific and precise. In comparison with the serum neutralization test it was superior in detecting low levels of antibodies in pig sera. The blocking ELISA gave higher titre values in serum samples taken early after experimental infection of pigs with Aujeszky's disease virus, than did the serum neutralization test.