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Generation of Murine Monoclonal Antibodies by Hybridoma Technology
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Generation of Murine Monoclonal Antibodies by Hybridoma Technology

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Affinity maturation and characterization of the ofatumumab monoclonal antibody

Zahra Payandeh1, Masoumeh Rajabibazl2,3, Yousef Mortazavi1,4

  • 1Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.

Journal of Cellular Biochemistry
|August 31, 2018
PubMed

CD20 molecule, a phosphoprotein with 297 amino acids and four transmembrane domains, is a member of MS4A protein family. Anti-CD20 antibodies such as ofatumumab, which have been developed for cancer treatment and has demonstrated efficacy in relapsed/refractory chronic lymphocytic leukemia, are among the most successful therapies to date. Rational engineering methods can be applied with reasonable success to improve functional characteristics of antibodies. Considering the importance of this issue, we have used in silico modeling approach for the improvement of ofatumumab monoclonal antibody. Four mutated variants of ofatumumab were developed and expressed in Chinese hamster ovary (CHO) cells along with the unmodified antibody. Analysis of affinity of the purified antibodies with CD20 showed significant improvement in antigen-binding characteristics of one of the variants compared with the control antibody. This study represents the first step toward development of the second generation ofatumumab antibody with improved affinity.

Keywords:
CD20affinity bindingchronic lymphocytic leukemiamonoclonal antibodiesofatumumab

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