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checkMyIndex: a web-based R/Shiny interface for choosing compatible sequencing indexes.

Hugo Varet1,2, Jean-Yves Coppée2

  • 1Center of Bioinformatics, Biostatistics and Integrative Biology, Institut Pasteur - Bioinformatics and Biostatistics Hub - C3BI, USR 3756 IP CNRS, Paris, France.

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Choosing compatible indexes is crucial for simultaneous sequencing. The new checkMyIndex tool helps researchers select optimal indexes based on their experimental needs, improving sequencing accuracy.

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Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • Simultaneous sequencing of multiple libraries requires careful selection of compatible indexes.
  • Indexes are short, sample-specific barcodes essential for distinguishing DNA fragments.
  • Current tools for selecting optimal indexes are limited, posing challenges for researchers.

Purpose of the Study:

  • To present checkMyIndex, an R/Shiny application designed to aid researchers in selecting compatible indexes.
  • To provide a user-friendly tool that simplifies the process of choosing indexes for multiplex sequencing experiments.

Main Methods:

  • Development of an online R/Shiny application named checkMyIndex.
  • The application facilitates the selection of indexes based on user-defined experimental constraints.

Main Results:

  • checkMyIndex offers a practical solution for researchers needing to select indexes for multiplex sequencing.
  • The tool aims to prevent sequencing errors caused by incompatible index combinations.

Conclusions:

  • checkMyIndex addresses a critical need in genomics research by providing a dedicated tool for index selection.
  • The application is freely available, promoting wider adoption and improved experimental outcomes in sequencing projects.