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Mesenchymal Stem Cell Functionalization for Enhanced Therapeutic Applications.

Dimitrios Kouroupis1,2, Clara Sanjurjo-Rodriguez3,4, Elena Jones3

  • 11 Department of Orthopedics, UHealth Sports Medicine Institute, University of Miami Miller School of Medicine, Miami, Florida.

Tissue Engineering. Part B, Reviews
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PubMed
Summary
This summary is machine-generated.

Mesenchymal stem cell (MSC) expansion alters cell traits, impacting therapy. Identifying distinct MSC subpopulations and optimizing ex vivo culture can improve therapeutic standardization and reproducibility.

Keywords:
MSC subpopulationscell primingcellular phenotypesmesenchymal stem cells

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Area of Science:

  • * Stem cell biology and regenerative medicine.

Background:

  • * In vitro culture of mesenchymal stem cells (MSCs) leads to detrimental changes in cell characteristics, including phenotypic shifts and senescence.
  • * Inter-donor variability further complicates the standardization and reproducibility of MSC therapeutic applications.
  • * Current limitations hinder the reliable clinical translation of MSC-based therapies.

Purpose of the Study:

  • * To address the limitations in MSC therapeutic potential caused by in vitro culture and donor variability.
  • * To explore strategies for maintaining MSC phenotypes and enhancing specific functions during ex vivo expansion.
  • * To identify innate distinct functional MSC subpopulations.

Main Methods:

  • * Investigated the effects of culture expansion on MSC characteristics.
  • * Analyzed inter-donor variability in MSC properties.
  • * Explored ex vivo protocols for preserving MSC phenotype and function.
  • * Characterized distinct functional MSC subpopulations.

Main Results:

  • * Culture expansion negatively impacts MSC phenotypic stability and promotes senescence.
  • * Significant inter-donor variability exists in MSC functional attributes.
  • * Identified specific ex vivo culture conditions to mitigate detrimental effects.
  • * Characterized distinct MSC subpopulations with unique functional profiles.

Conclusions:

  • * Strategies to maintain MSC phenotype and enhance function ex vivo are crucial for therapeutic standardization.
  • * Identifying and utilizing distinct functional MSC subpopulations can overcome current limitations.
  • * Implementing these approaches in cell-based therapies promises more reproducible clinical outcomes.