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Bactericidal effect of a diode laser on Enterococcus faecalis in human primary teeth-an in vitro study
Shanshan Dai1,2, Gang Xiao1,2, Ning Dong1,2
1Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, Xi'an, 710004, China.
BMC Oral Health
|September 1, 2018
Summary
Diode laser (810 nm) effectively disinfects root canals in primary teeth. Combining diode laser with sodium hypochlorite (NaOCl) achieved nearly 100% bacterial reduction, making it a superior endodontic therapy option.
Area of Science:
- Endodontics
- Dental Laser Applications
- Microbiology
Background:
- Diode lasers (810 nm) are increasingly utilized for root canal disinfection.
- Effective root canal disinfection is crucial for successful endodontic therapy.
Purpose of the Study:
- To evaluate the efficacy of a diode laser in disinfecting experimentally infected root canals of primary teeth.
- To compare the disinfecting ability of diode laser alone and in combination with sodium hypochlorite (NaOCl).
Main Methods:
- Primary teeth infected with Enterococcus faecalis were divided into four groups: no treatment, 5.25% NaOCl, diode laser, and diode laser with NaOCl.
- Bacterial counts, scanning electron microscopy, and confocal laser microscopy (live-dead staining) were used to assess disinfection efficacy.
Main Results:
- Diode laser irradiation and irrigation significantly reduced bacteria and smear layer compared to no treatment (p < 0.01).
- The combination of diode laser and NaOCl achieved nearly 100% bacterial reduction on root canal surfaces.
- Live bacteria were rarely observed in dentinal tubules with the diode-NaOCl group.
Conclusions:
- Diode laser is effective for disinfecting infected root canals in primary teeth.
- The combination of diode laser and NaOCl offers superior disinfection, approaching complete bacterial elimination.
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