Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Capillary Beds01:20

Capillary Beds

7.2K
Capillary beds are networks of tiny blood vessels that play a crucial role in the circulatory system. These beds are where the exchange of gases, nutrients, and waste products occurs between the blood and surrounding tissues. Each capillary bed consists of numerous capillaries, which are the smallest blood vessels in the body, typically only one cell-thick. This thinness allows for the efficient diffusion of substances.
Capillaries connect arterioles, small branches of arteries, to venules,...
7.2K
Operational Amplifiers01:17

Operational Amplifiers

2.0K
The operational amplifier, often referred to as an op-amp, is a multifaceted building block of a circuit. This electronic component functions like a voltage-controlled voltage source and can also be used to create a voltage- or current-controlled current source. The design of an operational amplifier enables it to execute mathematical operations when external components like resistors and capacitors are linked to its terminals. An op-amp has the capacity to sum signals, amplify a signal,...
2.0K
MOSFET Amplifiers01:17

MOSFET Amplifiers

521
The MOSFET, when operating in its active region, functions as a voltage-controlled current source. In this region, the gate-to-source voltage controls the drain current. This principle underlies the operation of the transconductance MOSFET amplifier. The output current is directed through a load resistor to convert this amplifier into a voltage amplifier. The output voltage is then obtained by subtracting the voltage drop across the load resistance from the supply voltage. This process results...
521
BJT Amplifiers01:14

BJT Amplifiers

995
Bipolar Junction Transistors (BJTs) are pivotal components in amplifier circuits, functioning as voltage-controlled current sources in their active region. This characteristic allows them to efficiently control the collector current through variations in the base-emitter voltage. Essentially, BJTs amplify power due to their ability to take a weak input signal and output a much stronger signal.
In BJT amplifier configurations, particularly in common-emitter setups, the transistor's role...
995
Instrumentation Amplifier01:25

Instrumentation Amplifier

1.1K
An electrocardiography (ECG) machine is an essential piece of medical equipment used to monitor the electrical activity of the heart. It operates by detecting small electrical changes on the skin that result from the depolarization of the heart muscle during each heartbeat. However, these signals are in the microvolt range and can be easily overwhelmed by noise or interference.
To overcome this challenge, an ECG machine utilizes an instrumentation amplifier. This specialized amplifier is...
1.1K
Amplifying Signals via Second Messengers01:15

Amplifying Signals via Second Messengers

8.8K
Many receptor binding ligands are hydrophilic; they do not cross the cell membrane but bind to cell-surface receptors. Thus, their message must be relayed by second messengers present in the cell cytoplasm. There are several second messenger pathways, each with its own way of relaying information. For example, the G protein-coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol pathway is active when the receptor induces...
8.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unforgeable Red: Design and Application of SO-Annulated Perylene Diimides for Anti-Counterfeiting.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Mechanisms by which proline reductase of <i>Clostridioides difficile</i> promotes efficient metabolism and disease progression <i>in vivo</i>.

mBio·2026
Same author

Generic optimization procedure for high-resolution printing by stereolithography of 3D scaffolds compatible for cell culture applied to a small intestinal architecture.

Biofabrication·2026
Same author

Co-enrichment of proteins in extracellular vesicles.

Nature communications·2026
Same author

In Vitro Measurement of Clostridioides difficile Biofilm Formation Induced by Gut and Microbiota-Derived Signals.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

An Integrative Network Analysis Framework for Identifying Altered Glycosylation Pathways Associated with Autism Spectrum Disorder.

Genes·2026

Related Experiment Video

Updated: Feb 5, 2026

Cell Capture Using a Microfluidic Device
29:02

Cell Capture Using a Microfluidic Device

Published on: October 1, 2007

5.7K

A microfluidic fluidized bed to capture, amplify and detect bacteria from raw samples.

Lucile Alexandre1, Iago Pereiro1, Amel Bendali1

  • 1Laboratoire Phyisico Chimie Curie, Institut Curie, PSL Research University, Paris, France; Sorbonne Universités, UPMC Univ Paris 06, Paris, France; Institut Pierre-Gilles de Gennes, Paris, France.

Methods in Cell Biology
|September 1, 2018
PubMed
Summary

This study introduces a new low-cost device for rapid, ultra-sensitive bacterial detection in complex samples. The technology enables early identification of infectious bacteria, crucial for public health and safety.

Keywords:
AmplificationBacteriaCaptureDetectionFluidized bedMagnetic beads

More Related Videos

Author Spotlight: Development of an Enhanced Protocol for Rapid and Accurate Isolation of Campylobacter from Food Products
05:34

Author Spotlight: Development of an Enhanced Protocol for Rapid and Accurate Isolation of Campylobacter from Food Products

Published on: February 23, 2024

2.7K
T Cells Capture Bacteria by Transinfection from Dendritic Cells
11:39

T Cells Capture Bacteria by Transinfection from Dendritic Cells

Published on: January 13, 2016

12.3K

Related Experiment Videos

Last Updated: Feb 5, 2026

Cell Capture Using a Microfluidic Device
29:02

Cell Capture Using a Microfluidic Device

Published on: October 1, 2007

5.7K
Author Spotlight: Development of an Enhanced Protocol for Rapid and Accurate Isolation of Campylobacter from Food Products
05:34

Author Spotlight: Development of an Enhanced Protocol for Rapid and Accurate Isolation of Campylobacter from Food Products

Published on: February 23, 2024

2.7K
T Cells Capture Bacteria by Transinfection from Dendritic Cells
11:39

T Cells Capture Bacteria by Transinfection from Dendritic Cells

Published on: January 13, 2016

12.3K

Area of Science:

  • Microfluidics
  • Biosensing
  • Bacteriology

Background:

  • Bacterial contamination poses a significant threat to human health, necessitating early detection in food, clinical, and environmental settings.
  • Current detection methods often require lengthy culture steps, delaying timely intervention.

Purpose of the Study:

  • To develop a low-cost, hand-held device for rapid, ultra-sensitive, and specific bacterial detection.
  • To enable a one-step detection process directly from complex raw samples within hours.

Main Methods:

  • Utilized a novel microfluidic magnetic fluidized bed for bacterial capture and detection.
  • Developed a one-step process with a detection time of 2-8 hours.
  • Achieved high sensitivity and quantification accuracy across a broad dynamic range.

Main Results:

  • Demonstrated ultra-sensitive detection with a limit of 4 colony forming units (CFU)/mL.
  • Achieved high quantification accuracy within a dynamic range of 100-10^7 CFU/mL.
  • Successfully detected specific bacterial strains, including Salmonella Typhimurium and E. coli O157:H15.
  • The method showed sensitivity exclusively to infectious bacteria, eliminating the need for prior culture steps.

Conclusions:

  • The novel microfluidic magnetic fluidized bed enables rapid, sensitive, and specific bacterial detection.
  • This technology offers a significant advancement for early threat limitation in food, clinical, and environmental safety.
  • The device's low cost and ease of use have broad application potential.