Hydrogen peroxide promotes gastric motility in the newborn rat

When compared with infant formula, human milk enhances gastric emptying in preterm infants. Hydrogen peroxide (H₂O₂) is present in large quantities in human milk that has an antimicrobial role for the mother and infant. In vitro adult rat studies suggest that H₂O₂ facilitates gastric motor contraction. Hypothesizing that H₂O₂ enhances gastric motility, we investigated its effects on the newborn rat stomach tissue.

Rat newborn and adult gastric fundic segments, or their smooth muscle cells, were used to evaluate the muscle response to H₂O₂ exposure. Tissue expression of Rho kinase 2 (ROCK-2; Western blot), its catalase activity, and H₂O₂ content (Amplex Red) were measured. H₂O₂ gastric mucosal diffusion was evaluated with Ussing chambers.

In both newborn and adult rats, H₂O₂ induced gastric muscle contraction and this response was attenuated by pre-incubation with the antioxidant melatonin. H₂O₂ passively diffused across the gastric mucosa. Its effect on the muscle was modulated via ROCK-2 activation and inhibited by melatonin.

H₂O₂, at a concentration similar to that of human milk, promotes gastric motility in the rat. To the extent that the present findings can be clinically extrapolated, the human milk H₂O₂ content may enhance gastric emptying in neonates.