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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Atomic Force Microscopy01:08

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Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
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An atomic orbital represents the three-dimensional regions in an atom where an electron has the highest probability to reside. The radial distribution function indicates the total probability of finding an electron within the thin shell at a distance r from the nucleus. The atomic orbitals have distinct shapes which are determined by l, the angular momentum quantum number. The orbitals are often drawn with a boundary surface, enclosing densest regions of the cloud.
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Atoms and molecules interact through bonds (or forces): intramolecular and intermolecular. The forces are electrostatic as they arise from interactions (attractive or repulsive) between charged species (permanent, partial, or temporary charges) and exist with varying strengths between ions, polar, nonpolar, and neutral molecules. The different types of intermolecular forces are ion–dipole, dipole–dipole, hydrogen bonds, and dispersion; among these, dipole–dipole, hydrogen...
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In an atom, the negatively charged electrons are attracted to the positively charged nucleus. In a multielectron atom, electron-electron repulsions are also observed. The attractive and repulsive forces are dependent on the distance between the particles, as well as the sign and magnitude of the charges on the individual particles. When the charges on the particles are opposite, they attract each other. If both particles have the same charge, they repel each other.
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Intermolecular forces (IMF) are electrostatic attractions arising from charge-charge interactions between molecules. The strength of the intermolecular force is influenced by the distance of separation between molecules. The forces significantly affect the interactions in solids and liquids, where the molecules are close together. In gases, IMFs become important only under high-pressure conditions (due to the proximity of gas molecules). Intermolecular forces dictate the physical properties of...
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Super-resolution visible photoactivated atomic force microscopy.

Seunghyun Lee1, Owoong Kwon2, Mansik Jeon1,3

  • 1Future IT Innovation Laboratory, Department of Creative IT Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea.

Light, Science & Applications
|September 1, 2018
PubMed
Summary
This summary is machine-generated.

Super-resolution visible photoactivated atomic force microscopy (pAFM) images intrinsic optical absorption with ~8 nm resolution. This technique offers a cost-effective, non-destructive alternative to electron microscopy for nanomaterial and biological cell imaging.

Keywords:
Arabidopsis imaginggold nanoparticle imagingmelanoma cell imagingnanowire imagingsuper-resolution optical microscopy

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Area of Science:

  • Nanotechnology
  • Optical Microscopy
  • Surface Science

Background:

  • Optical microscopy (OM) faces limitations in resolving intrinsic optical absorption of nanomaterials due to the diffraction limit and poor sensitivity.
  • Electron microscopy (EM) is often used for nanostructure morphology but is expensive and destructive.
  • Nanoscale fluorescence OM is unsuitable for optical absorption imaging and may require exogenous labels for biological samples.

Purpose of the Study:

  • To develop a super-resolution imaging technique for intrinsic optical absorption of nanomaterials.
  • To overcome the limitations of conventional optical and electron microscopy for nanoscale absorption imaging.
  • To provide a cost-effective and non-destructive method for high-resolution imaging.

Main Methods:

  • Demonstration of super-resolution visible photoactivated atomic force microscopy (pAFM).
  • Utilizing nonlinear effects to detect first and higher harmonic responses.
  • Employing thermoelastic effects induced by pulsed laser irradiation for imaging.

Main Results:

  • Achieved ~8 nm resolution for imaging intrinsic optical absorption.
  • Successfully imaged single gold nanospheres, nanowires, and biological cells with nanoscale resolution.
  • Developed a system that can be added to commercial atomic force microscopes.

Conclusions:

  • Visible pAFM provides super-resolution imaging of intrinsic optical absorption.
  • This technique offers a simpler and more affordable alternative to EM for nanoscale imaging.
  • The method is applicable to diverse nanostructures and biological specimens.