Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A common plasmid of Chlamydia trachomatis.

L Palmer, S Falkow

    Plasmid
    |July 1, 1986
    PubMed
    Summary

    The Chlamydia trachomatis plasmid, a common genome component, is essential for intracellular growth. Its genes are expressed in vivo and in vitro, suggesting a vital role in chlamydial pathogenesis.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Protein domain movement involved in binding of belinostat and HPOB as inhibitors of histone deacetylase 6 (HDAC6): a hybrid automated-interactive docking study.

    Journal of computer-aided molecular design·2025
    Same author

    Understanding HCBS utilization: The role of disability onset age and length of disability.

    Disability and health journal·2025
    Same author

    Place-based household vouchers for locally supplied fruit and vegetables: the Fresh Street pilot cluster randomised controlled trial.

    BMC public health·2025
    Same author

    Breakthrough cancer pain management: mixed-methods study of health care professionals.

    BMJ supportive & palliative care·2024
    Same author

    A systematic review of qualitative research exploring patient and health professional perspectives of breakthrough cancer pain.

    Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer·2023
    Same author

    Synovial sepsis is rare following intrasynovial medication in equine ambulatory practice.

    Equine veterinary journal·2018

    Area of Science:

    • Microbiology
    • Genomics
    • Molecular Biology

    Background:

    • The 7.4-kb plasmid is a common component of the Chlamydia trachomatis genome, present in approximately 10 copies per chromosomal equivalent.
    • This plasmid is crucial for the intracellular growth of Chlamydia trachomatis.

    Purpose of the Study:

    • To characterize the Chlamydia trachomatis plasmid.
    • To investigate the expression of plasmid-encoded genes.
    • To determine the plasmid's role in intracellular growth.

    Main Methods:

    • Isolation and cloning of complete plasmid genomes from eight C. trachomatis serovars into E. coli.
    • Restriction enzyme cleavage site mapping to assess plasmid relatedness.
    • DNA hybridization to detect homologous sequences in clinical isolates.
    • Gene expression analysis in E. coli minicells and in vitro transcription-translation systems.

    Main Results:

    • Complete plasmid genomes were successfully cloned and mapped, revealing close genetic relatedness among different serovars.
    • Homologous plasmid sequences were detected in all 200 clinically isolated C. trachomatis strains examined.
    • No homologous sequences were found in eukaryotic DNA or in a similar-sized plasmid from C. psittaci.
    • Plasmid-encoded genes were expressed both in vivo and in vitro, indicating functional gene products.

    Conclusions:

    • The Chlamydia trachomatis plasmid is a conserved and essential element of the organism's genome.
    • Plasmid-encoded genes are expressed and likely contribute to the intracellular survival and growth of Chlamydia trachomatis.
    • The plasmid represents a potential target for therapeutic interventions against Chlamydia trachomatis infections.

    Related Experiment Videos