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Updated: Feb 5, 2026

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
Published on: June 15, 2016
Transcript Level of MicroRNA Processing Elements in Gastric Cancer
Milad Asadi1,2, Dariush Shanehbandi2, Venus Zafari3
1Research Centers for Liver and Gastrointestinal Disease, Tabriz University of Medical Sciences, Tabriz, Iran.
Gastric cancer tissues show increased levels of Drosha, Dicer, and DGCR8 mRNA. These microRNA biosynthesis genes are upregulated in tumors, suggesting a role in cancer development.
Area of Science:
- Molecular Biology
- Oncology
- Gene Expression Analysis
Background:
- MicroRNAs (miRNAs) are crucial in cancer development.
- Drosha, Dicer, and DGCR8 are key enzymes in miRNA biosynthesis.
- Aberrant miRNA expression is linked to various cancers, including gastric cancer.
Purpose of the Study:
- To evaluate the mRNA transcript levels of Drosha, Dicer, and DGCR8 genes in gastric cancer tissues.
- To investigate the potential role of these miRNA biogenesis genes in gastric carcinogenesis.
Main Methods:
- Real-time PCR was used to quantify mRNA expression.
- SYBR Green master mix was utilized for gene expression analysis.
- Tumoral and marginal tissues from 50 gastric cancer patients were analyzed.
Main Results:
- mRNA expression of Drosha, Dicer, and DGCR8 was significantly upregulated in tumoral tissues compared to marginal tissues.
- No correlation was found between the upregulation of these genes and clinical manifestations.
- These findings suggest a specific role for these genes in gastric cancer.
Conclusions:
- Upregulation of Drosha, Dicer, and DGCR8 is implicated in gastric cancer development.
- Dysregulated expression of these miRNA biogenesis genes may contribute to altered miRNA levels in cancer.
- Further research is warranted to elucidate the precise mechanisms.
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