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Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
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Transcript Level of MicroRNA Processing Elements in Gastric Cancer

Milad Asadi1,2, Dariush Shanehbandi2, Venus Zafari3

  • 1Research Centers for Liver and Gastrointestinal Disease, Tabriz University of Medical Sciences, Tabriz, Iran.

Journal of Gastrointestinal Cancer
|September 1, 2018
PubMed
Abstract

Insights

Gastric cancer tissues show increased levels of Drosha, Dicer, and DGCR8 mRNA. These microRNA biosynthesis genes are upregulated in tumors, suggesting a role in cancer development.

Area of Science:

  • Molecular Biology
  • Oncology
  • Gene Expression Analysis

Background:

  • MicroRNAs (miRNAs) are crucial in cancer development.
  • Drosha, Dicer, and DGCR8 are key enzymes in miRNA biosynthesis.
  • Aberrant miRNA expression is linked to various cancers, including gastric cancer.

Purpose of the Study:

  • To evaluate the mRNA transcript levels of Drosha, Dicer, and DGCR8 genes in gastric cancer tissues.
  • To investigate the potential role of these miRNA biogenesis genes in gastric carcinogenesis.

Main Methods:

  • Real-time PCR was used to quantify mRNA expression.
  • SYBR Green master mix was utilized for gene expression analysis.
  • Tumoral and marginal tissues from 50 gastric cancer patients were analyzed.

Main Results:

  • mRNA expression of Drosha, Dicer, and DGCR8 was significantly upregulated in tumoral tissues compared to marginal tissues.
  • No correlation was found between the upregulation of these genes and clinical manifestations.
  • These findings suggest a specific role for these genes in gastric cancer.

Conclusions:

  • Upregulation of Drosha, Dicer, and DGCR8 is implicated in gastric cancer development.
  • Dysregulated expression of these miRNA biogenesis genes may contribute to altered miRNA levels in cancer.
  • Further research is warranted to elucidate the precise mechanisms.
Keywords:
DGCR8DicerDroshaGastric cancerMicroRNA

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