Related Experiment Video
Updated: Feb 5, 2026

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping
Published on: May 25, 2015
A SimpleProbe® real-time PCR assay for differentiating the canine parvovirus type 2 genotype
Minh Hoang1,2, Hung-Yi Wu3, Ying-Xiu Lien4
1Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan.
Background:
Canine parvovirus type 2 (CPV-2) causes an important canine viral disease worldwide. CPV-2 belongs to the Protoparvovirus genus in the family Parvoviridae. An amino acid change at position 426 of the VP2 protein differentiate types of CPV-2, designated as CPV-2a (Asn), CPV-2b (Asp), and CPV-2c (Glu). In this study, we compared CPV-2 genotyping results obtained by SimpleProbe® real-time PCR and DNA sequencing analysis to identify the accuracy and sensitivity of these methods.
Methods:
One hundred rectal swabs were collected from CPV-2 naturally infected dogs from 2015 to 2017 at the Animal Disease Diagnostic Center, National Pingtung University of Science and Technology. CPV-2 genotyping was performed by SimpleProbe® real-time PCR and DNA sequencing to compare results.
Results:
CPV-2a (n = 23), 2b (n = 6) and 2c (n = 71) genotyping results obtained by both techniques were identical with specificity of 100% for SimpleProbe® assay. In the SimpleProbe® assay, amplifying the DNAs prepared from the clinical specimens showed three distinct melting curve peaks. CPV-2b had the highest melting peak of 57.8°C (CI 95%: 57.7-58.5°C) followed by CPV-2c with a slightly lower melting peak of 52.3°C (CI 95%: 52.2-53.2°C) and CPV-2a with the lowest peak of 50.2°C (CI 95%: 50.1-50.5°C).
Conclusion:
This study developed a novel method for genotyping CPV-2 strains using the SimpleProbe® real-time PCR assay. This assay is a reliable and sensitive tool for differentiating between the CPV-2a, 2b and 2c and this technique can be used for molecular CPV-2 epidemiology studies.
More Related Videos
06:18Frequency and Distribution of Crossovers in Caenorhabditis elegans Meiosis by SNP Genotyping using Real-time PCR
Published on: July 11, 2025
10:00High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
Published on: March 24, 2015
Related Concept Videos
Real Time RT-PCR
The real-time quantification of the number of amplified products is...
Diabetes Mellitus: Type 2 and Gestational
PCR
Real Number System
meta-Directing Deactivators: –NO2, –CN, –CHO, –⁠CO2R, –COR, –CO2H
Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...