Synthesis and characterization of various 5'-dye-labeled ribonucleosides
Coralie De Schutter1, Vincent Roy, Patrick Favetta
1Univ. Orléans, CNRS, ICOA, UMR 7311, F-45067 Orléans, France. luigi.agrofoglio@univ-orleans.fr.
Abstract:
Hitherto unknown chromophoric nucleosides are reported. This novel set of visibly coloured dye-labeled 5'-nucleosides, including 1,2,4,5-tetrazine, dicyanomethylene-4H-pyran, benzophenoxazinone, 9,10-anthraquinone and azobenzene chromophores, were prepared mainly under Cu-catalyzed azide-alkyne cycloaddition (CuAAC). The design criteria are outlined. Several derivatives possess in supplement a fluorescence property. The absorption and fluorescence spectra of all coloured nucleosides were recorded to study their potential as visible-range probes. Such nucleodyes are of great interest for future competitive lateral flow test MIP-based strips.
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