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Localization of multidrug resistance-associated DNA sequences to human chromosome 7
Somatic Cell and Molecular Genetics
|July 1, 1986
Abstract:
Multidrug resistance in several human cell lines correlates with amplification or increased expression of two related DNA sequences, designated mdr1 and mdr2. These DNA sequences were used as probes for hybridization with DNA with a panel of human-mouse somatic cell hybrids and from individual human chromosomes separated by fluorescence-activated chromosome sorting. By these assays, both mdr1 and mdr2 sequences were localized to chromosome 7.
Insights
Multidrug resistance genes mdr1 and mdr2 are linked to chromosome 7 in human cells. This finding helps understand the genetic basis of drug resistance.
Area of Science:
- Genetics
- Molecular Biology
- Cell Biology
Background:
- Multidrug resistance (MDR) in human cell lines is associated with amplified or overexpressed DNA sequences.
- Two related DNA sequences, mdr1 and mdr2, are implicated in MDR.
Purpose of the Study:
- To determine the chromosomal location of the mdr1 and mdr2 DNA sequences.
- To understand the genetic underpinnings of multidrug resistance.
Main Methods:
- Hybridization assays using DNA from human-mouse somatic cell hybrids.
- Analysis of DNA from individual human chromosomes sorted by fluorescence-activated cell sorting (FACS).
Main Results:
- Both mdr1 and mdr2 DNA sequences were successfully localized.
- The assays definitively mapped both mdr1 and mdr2 sequences to human chromosome 7.
Conclusions:
- The genetic loci for mdr1 and mdr2 are located on chromosome 7.
- This localization provides a basis for further investigation into the role of chromosome 7 in multidrug resistance mechanisms.