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Assembly factors in poliovirus morphogenesis.

B Rombaut, R Vrijsen, A Boeye

    Virology
    |August 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Poliovirus extracts promote empty capsid assembly. Iodoacetamide treatment inactivates this, but heat-disrupted virions restore assembly with altered antigenicity, distinguishing assembly and antigenicity roles.

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    Area of Science:

    • Virology
    • Molecular Biology
    • Cell Biology

    Background:

    • Poliovirus replication involves the assembly of viral capsids.
    • HeLa cells are commonly used for poliovirus studies.
    • Understanding capsid assembly is crucial for viral pathogenesis research.

    Purpose of the Study:

    • To investigate the factors responsible for poliovirus empty capsid assembly in vitro.
    • To differentiate between assembly-promoting and antigenicity-conferring activities in infected cell extracts.

    Main Methods:

    • Utilized extracts from poliovirus-infected HeLa cells for in vitro assembly assays.
    • Employed iodoacetamide treatment to inactivate specific activities.
    • Introduced heat-disrupted virions to assess restoration of assembly and antigenicity.

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    Main Results:

    • Infected cell extracts promoted in vitro assembly of 14 S subunits into empty capsids resembling procapsids.
    • Iodoacetamide treatment abolished assembly-promoting activity.
    • Restoration with heat-disrupted virions yielded capsids with different antigenic properties.

    Conclusions:

    • A distinction exists between assembly-promoting and antigenicity-conferring activities in poliovirus-infected cell extracts.
    • These activities can be modulated independently, suggesting distinct molecular mechanisms.