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Flow Cytometry01:23

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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When a paint brush is immersed in water, the bristles wave freely inside the water. When it is taken out, the bristles stick together. The reason behind this effect is surface tension.
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The various IMFs between identical molecules of a substance are examples of cohesive forces. The molecules within a liquid are surrounded by other molecules and are attracted equally in all directions by the cohesive forces within the liquid. However, the molecules on the surface of a liquid are attracted only by about one-half as many molecules. Because of the unbalanced molecular attractions on the surface molecules, liquids contract to form a shape that minimizes the number...
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Wood surfacing is a critical finishing process designed to smoothen the wood surface, enhance its dimensional accuracy, and make handling safer. This process compensates for potential shrinkage during the seasoning phase by marginally increasing the wood dimensions before surfacing. It also helps correct some distortions that may occur as the wood dries.
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Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence
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Flow cytometry: Surface markers and beyond.

Ottavia M Delmonte1, Thomas A Fleisher2

  • 1Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), Bethesda, Md.

The Journal of Allergy and Clinical Immunology
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Summary

Flow cytometry is a versatile lab technique for analyzing cells. Its expanded applications now aid in diagnosing immune system disorders by assessing immune cell function.

Keywords:
Immunophenotypeflow cytometryimmune function testingprimary immunodeficiency

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Area of Science:

  • Immunology
  • Clinical Laboratory Science

Background:

  • Flow cytometry is a standard laboratory technique for analyzing cells in suspension.
  • Its clinical applications have expanded beyond cell-surface protein identification.

Purpose of the Study:

  • To highlight the evolution and expanded utility of flow cytometry in clinical diagnostics.
  • To emphasize its role in assessing immune function for disease characterization.

Main Methods:

  • Utilizes flow cytometry to analyze cells from various human sources.
  • Characterizes both cell-surface and intracellular proteins.
  • Employs multiple techniques to assess adaptive and innate immune function.

Main Results:

  • Flow cytometry has evolved from basic cell identification to complex immune function analysis.
  • The expanded testing capabilities enhance its diagnostic utility.

Conclusions:

  • Flow cytometry is an increasingly valuable tool for characterizing and diagnosing immune function disorders.
  • Its broad applications support advancements in clinical immunology and laboratory diagnostics.