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Isolation of Primary Murine Brain Microvascular Endothelial Cells
08:14

Isolation of Primary Murine Brain Microvascular Endothelial Cells

Published on: November 14, 2014

34.0K

Effect of tryptase on mouse brain microvascular endothelial cells via protease-activated receptor 2

Qin Zhou1, Yi-Wei Wang1, Peng-Fei Ni1

  • 1Department of Anesthesiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, Jiangsu, People's Republic of China.

Journal of Neuroinflammation
|September 2, 2018
PubMed
Abstract

Insights

Mast cells release tryptase, which activates brain endothelial cells via PAR-2, disrupting the blood-brain barrier (BBB). This study clarifies tryptase

Area of Science:

  • Neuroscience
  • Immunology
  • Cell Biology

Background:

  • Mast cells (MCs) are key in brain injury, potentially disrupting the blood-brain barrier (BBB).
  • Tryptase, an abundant MC product, interacts with Protease-activated receptor 2 (PAR-2) on brain microvascular endothelial cells, crucial for BBB integrity.
  • The precise mechanism of MC-induced BBB disruption requires elucidation.

Purpose of the Study:

  • To investigate the effects of tryptase on mouse brain microvascular endothelial cells (bEnd3).
  • To explore the potential mechanisms underlying tryptase-induced changes in these cells.

Main Methods:

  • Examined tryptase-induced activation of mouse brain microvascular endothelial cells.
  • Assessed cellular activation, cytokine production, and pathway signaling (MAPK, NF-κB) after PAR-2 antagonism and tryptase stimulation.

Main Results:

  • Tryptase increased VCAM-1, MMPs, TLR4, and TNF-α, while decreasing tight junction proteins (occludin, claudin-5).
  • Tryptase activated ERK and NF-κB signaling pathways.
  • PAR-2 inhibition abolished these tryptase-induced effects.

Conclusions:

  • Tryptase activates brain microvascular endothelial cells and promotes pro-inflammatory mediator release.
  • These findings elucidate the mechanism of tryptase in blood-brain barrier disruption.
Keywords:
Brain microvascular endothelial cellsMAPKNF-kappa BProtease-activated receptor 2 (PAR-2)Tryptase

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