Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

28.1K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
28.1K
Tissue Renewal without Stem Cells01:23

Tissue Renewal without Stem Cells

2.1K
After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...
2.1K
Adult Stem Cells01:33

Adult Stem Cells

33.9K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
33.9K
Stem Cell Therapy for Tissue Regeneration01:21

Stem Cell Therapy for Tissue Regeneration

4.7K
Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
4.7K
Plant Cells and Tissues02:01

Plant Cells and Tissues

65.8K
Plant tissues are collections of similar cells performing related functions. Different plant tissues will have their own specialized roles and can be combined with other tissues to form organs such as flowers, fruit, stem, and leaves. Two major types of plant tissue include meristematic and permanent tissue.
65.8K
Embryonic Stem Cells00:58

Embryonic Stem Cells

32.5K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
32.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-cell multi-omic atlas and morphogen screening informs midbrain and hindbrain organoid engineering.

Nature neuroscience·2026
Same author

Engineering Neuronal Network Connectivity Through Precise and Scalable Electrical Modulation.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Unlocking the full potential of nanopore sequencing: tips, tricks, and advanced data analysis techniques.

Nucleic acids research·2026
Same author

Integrated Microfluidic Platform for High-Throughput Generation of Intestinal Organoids in Hydrogel Droplets.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Systematic scRNA-seq screens profile neural organoid response to morphogens.

Nature methods·2025
Same author

Recent evolution of the developing human intestine affects metabolic and barrier functions.

Science (New York, N.Y.)·2025

Related Experiment Video

Updated: Feb 5, 2026

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9
08:20

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Published on: November 3, 2020

7.0K

Single-cell genomics to guide human stem cell and tissue engineering.

J Gray Camp1, Damian Wollny2, Barbara Treutlein3,4,5

  • 1Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany. gray_camp@eva.mpg.de.

Nature Methods
|September 2, 2018
PubMed
Summary
This summary is machine-generated.

Single-cell genomics (SCG) advances human cell and tissue engineering by precisely measuring accuracy and detecting inefficiencies. Emerging SCG methods also help reverse-engineer human tissues and unravel disease mechanisms.

More Related Videos

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells
12:04

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

Published on: March 10, 2023

4.9K
Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
09:51

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Published on: February 2, 2016

14.2K

Related Experiment Videos

Last Updated: Feb 5, 2026

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9
08:20

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Published on: November 3, 2020

7.0K
Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells
12:04

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

Published on: March 10, 2023

4.9K
Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
09:51

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Published on: February 2, 2016

14.2K

Area of Science:

  • Biotechnology
  • Genomics
  • Regenerative Medicine

Background:

  • Understanding human development and disease requires physiologically relevant in vitro models.
  • Engineering cells and 3D microenvironments is crucial for creating these models.
  • Single-cell genomics (SCG) offers high-resolution insights into cellular diversity.

Purpose of the Study:

  • To discuss the application of SCG in optimizing human cell and tissue engineering.
  • To explore how SCG can be used to understand disease mechanisms.
  • To provide a perspective on emerging SCG methods for tissue engineering and disease research.

Main Methods:

  • Application of single-cell genomics technologies to primary human organs.
  • Utilizing SCG to analyze engineered cells and tissues.
  • Leveraging SCG for precision measurement, inefficiency detection, and accuracy assessment in engineering.

Main Results:

  • SCG generates high-resolution atlases of cell diversity in human organs and engineered systems.
  • SCG methods provide powerful tools for gaining insights into engineering and disease processes.
  • Emerging SCG methods show potential for reverse-engineering human cells and tissues.

Conclusions:

  • Single-cell sequencing is instrumental in optimizing human cell and tissue engineering.
  • SCG can elucidate complex biological processes and disease mechanisms.
  • Future applications of SCG hold promise for advancing regenerative medicine and disease understanding.