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Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells
Published on: December 3, 2015
Differentiation of Human Pluripotent Stem Cells to Megakaryocytes by Transcription Factor-Driven Forward Programming
Thomas Moreau1, Amanda L Evans2, Cedric J G Ghevaert3
1Department of Haematology, Wellcome Trust - Medical Research Council Cambridge Stem Cell Institute, NHS Blood and Transplant, Long Road, University of Cambridge, Cambridge, UK. tm384@cam.ac.uk.
Abstract:
The differentiation of megakaryocytes from human pluripotent stem cells in vitro offers intriguing new perspectives for research and transfusion medicine. However, applications have been hampered by the low efficiency of cytokine driven differentiation protocols leading to poor megakaryocyte purity and yield. Here we describe a novel forward programming approach relying on the combined ectopic expression of the three transcription factors GATA1, FLI1, and TAL1 in human pluripotent stem cells for large scale production of mature megakaryocytes using chemically defined culture and minimum cytokines.
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