Metal internalization by bacterial cells depends on metal biotoxicity and metal to biomass ratio

The traditional view of metal adsorption to bacterial surfaces is that it can act as a protective mechanism by externalizing the metal outside the cell. However, numerous studies focussing on the biodynamics of metal uptake using biotic ligand models consider metal adsorption to cell surfaces as an important first step in metal uptake and internalization. In order to resolve these conflicting views, we adsorbed two metals (copper and cadmium) with contrasting metal biotoxicity on E. coli JM109, and quantified the distribution of each metal amongst surface sites, periplasmic space and the cytoplasm. Distribution of each metal depended on biotoxicity and metal to biomass ratio. For both metals, low metal to biomass ratio led to most of the metal being associated with the periplasmic space, with less Cd being taken up by cells overall. At high metal to biomass ratios, most of the Cd was associated with surface sites, whereas Cu also increased in surface sites but remained below periplasmic concentrations. These observations are consistent with metal internalization being the dominant process at low metal to biomass ratios, whereas was active efflux when metal to biomass was high, leading to equilibrium between cytoplasm and surface concentrations. Significantly, efflux was more intense for high biotoxicity Cd, consistent with active enzymatic regulation of Cu internalization/homeastasis, which is essential at low concentrations. Moreover, metal internalization increases as surface-bound metal increases, the maximum being constrained by maximum adsorption consistent with Langmuir adsorption behaviour.

Bacterial metal internalization is a function of metal biotoxicity and metal loading.