Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Difference from Background: Limit of Detection01:05

Difference from Background: Limit of Detection

8.0K
The limit of detection (LOD) is the smallest amount of analyte that can be distinguished from the background noise. The LOD value corresponds to the concentration at which the analyte signal is three times larger than the standard deviation of the blank signal. Below this value, the analyte signal cannot be differentiated from the background noise. It is calculated by dividing the calibration slope by 3 times the standard deviation of the blank signals.
The LOD indicates the presence or absence...
8.0K
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

1.5K
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
1.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Focusing performance of Fibonacci tiling-based zone plates.

Scientific reports·2026
Same author

Shedding Light on Halos: Quantifying the Impact of the Diffractive Profile in Multifocal Intraocular Lenses.

Translational vision science & technology·2026
Same author

Corrigendum to "Environmental monitoring of a climate change indicator (Vibrio vulnificus) in coastal wetland water samples based on field-deployable detection [Sci. Total Environ. 986: 179791]".

The Science of the total environment·2025
Same author

Reliability of Prediction Models for the Functional Classification of a Sinusoidal Intraocular Lens Depending on Pupil Diameter.

Diagnostics (Basel, Switzerland)·2025
Same author

Drying-Induced Salt Deposition Patterns as a Tool for Label-Free Protein Quantification.

Biosensors·2025
Same author

Synthetic biology-driven optoelectronic biosensor for rapid and highly sensitive norovirus detection in fecal samples.

Biosensors & bioelectronics·2025

Related Experiment Video

Updated: Jan 13, 2026

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
10:10

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

9.4K

A label-free diffraction-based sensing displacement immunosensor to quantify low molecular weight organic compounds.

Miquel Avella-Oliver1, Vicente Ferrando2, Juan A Monsoriu2

  • 1Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, 46022, Valencia, Spain.

Analytica Chimica Acta
|September 3, 2018
PubMed
Summary

A novel diffractometric immunosensor detects low molecular weight organic compounds without labels. This pesticide sensor achieves a 1.1 ng/mL detection limit for atrazine, enabling sensitive environmental analysis.

Keywords:
AtrazineBiogratingDiffraction-based sensingDisplacement immunoassayLabel-freeMicrocontact printing

More Related Videos

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

10.2K
Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

12.6K

Related Experiment Videos

Last Updated: Jan 13, 2026

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
10:10

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

9.4K
Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

10.2K
Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

12.6K

Area of Science:

  • Analytical Chemistry
  • Biosensing Technology
  • Environmental Science

Background:

  • Low molecular weight organic compounds, such as pesticides, pose environmental and health risks.
  • Accurate and sensitive detection methods are crucial for monitoring these compounds.
  • Existing methods often require labeling or complex procedures, limiting field applicability.

Purpose of the Study:

  • To develop a label-free diffractometric immunosensor for quantifying low molecular weight organic compounds.
  • To fabricate and optimize diffractive structures for biological probes.
  • To apply the sensor for the detection of atrazine, a widely used pesticide.

Main Methods:

  • Patterning analyte analogues (haptens) on solid surfaces with diffractive structures.
  • Immobilizing specific antibodies onto the patterned surfaces.
  • Measuring changes in diffractive response upon antibody displacement by free analytes in solution.
  • Assessing the sensor's performance for atrazine detection.

Main Results:

  • Successful fabrication and optimization of diffractive biological probe structures.
  • Demonstrated label-free quantification of atrazine with high sensitivity.
  • Achieved a detection limit of 1.1 ng/mL for atrazine.
  • Obtained well-correlated dose-response curves.

Conclusions:

  • The developed diffractometric immunosensor offers a simple and sensitive method for label-free quantification of organic compounds.
  • This technology shows potential for in-field analysis and screening of multiple analytes.
  • The approach provides a foundation for developing advanced sensing strategies for environmental monitoring.