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Updated: Feb 5, 2026

Application of Membrane and Cell Wall Selective Fluorescent Dyes for Live-Cell Imaging of Filamentous Fungi
Published on: November 28, 2019
A simple pyrene-based fluorescent probe for highly selective detection of formaldehyde and its application in
Di Zhang1, Dongmei Liu1, Man Li1
1Institute of Quality Standards and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China; Henan Key Laboratory of Grain Quality and Safety and Testing, Zhengzhou, 450002, China; Laboratory of Quality & Safety Risk Assessment for Agro-Products (Zhengzhou), Ministry of Agriculture, Zhengzhou, 450002, China.
Abstract:
A novel and simple pyrene-based fluorescent probe (B1) was synthesized and used for the detection of formaldehyde (FA). When B1 was added to FA in CH3CH2OH/HEPES (10 mM, pH = 7.4, 1:99, v/v) solution, the solution system strongly enhanced the fluorescence. Over various small molecular species, probe B1 showed high sensitivity and selectivity for detecting FA, with a low limit of detection (0.107 μM). Furthermore, probe B1 was successfully applied for visualizing FA in human embryonic kidney (HEK293T) cells, demonstrating its value of application in living biomedical samples.
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