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Electrochromatographic behavior of core-shell particles: A comparison study.

Lijun Yang1, Lingjia Xu1, Rui Guo1

  • 1Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.

Analytica Chimica Acta
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Summary

Core-shell particles in liquid chromatography offer improved peptide and protein separation. While showing lower electroosmotic flow, their high retentivity enhances resolution for challenging hydrophilic compounds.

Keywords:
Capillary column technologyCore-shell particleElectrochromatographyFused-core technologySingle particle fritSuperficially porous particle

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Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Core-shell particles represent advanced packing materials for high-performance liquid chromatography.
  • Traditional totally porous silica particles are widely used but have limitations in certain separation scenarios.

Purpose of the Study:

  • To evaluate the electrochromatographic behavior of Ascentis Express 5 μm core-shell particles.
  • To compare their performance against totally porous silica particles of similar size.
  • To assess their suitability for bioanalytical applications, including peptide and protein analysis.

Main Methods:

  • Comparative electrochromatographic analysis of core-shell and totally porous silica particles.
  • Assessment of voltage-current properties, electroosmotic flow (EOF), and van Deemter curves.
  • Evaluation of separation performance for protein digests and hydrophilic peptides.

Main Results:

  • Core-shell particles exhibited reduced EOF and efficiency compared to totally porous particles due to their non-permeable core.
  • This observation confirmed the significant role of intra-particle flow in totally porous materials.
  • Enhanced retentivity of core-shell particles improved resolution for poorly retained hydrophilic peptides.
  • Efficient separation of protein digests was achieved with excellent resolution, reproducibility, and stability.

Conclusions:

  • The solid core in core-shell particles influences electrochromatographic properties, notably reducing EOF and efficiency.
  • Core-shell particles demonstrate superior performance for specific applications like separating weakly retained hydrophilic peptides.
  • The core-shell design is a promising stationary phase for bioelectrochromatography of peptides and proteins, offering high resolution and stability.