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Updated: Feb 5, 2026

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues
Published on: May 4, 2022
Core-shell structured magnetic metal-organic framework composites for highly selective enrichment of endogenous
Yonglei Wu1, Qianjing Liu1, Yiqin Xie1
1Department of Chemistry, Fudan University, Shanghai 200433, China.
Abstract:
In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand. The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC. Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples.
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